|
| Status |
Public on Jun 11, 2019 |
| Title |
WtPH: Vector + TNFa 3 |
| Sample type |
RNA |
| |
|
| Source name |
primary hepatocytes
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6J
tissue: liver
cell line: WtPH
genotype/variation: Control (baseline)
cell type: primary mouse hepatocytes
isoform: Control (vector)
treatment: TNFalpha
|
| Treatment protocol |
Adenoviral infection (48 hours) followed by treatment for two hours with 2 ng/mL TNFalpha
|
| Growth protocol |
25 mM glucose DMEM supplemented with 0,2% BSA, 2 mM sodium pyruvate, and pen/strep.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy Kit column extraction
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
standard Affymetrix procedure.
|
| Scan protocol |
standard Affymetrix procedure.
|
| Description |
microarray expression data from primary hepatocytes
|
| Data processing |
The data were analyzed with RMA (log2) normalized data using the just.rma function in the affy package in Biocinductor
|
| |
|
| Submission date |
Jun 10, 2019 |
| Last update date |
Jun 11, 2019 |
| Contact name |
Jennifer Lynn Estall |
| E-mail(s) |
jennifer.estall@ircm.qc.ca
|
| Phone |
514-987-5688
|
| Organization name |
IRCM
|
| Street address |
110 avenue des Pins ouest
|
| City |
Montreal |
| State/province |
Quebec |
| ZIP/Postal code |
H2W1R7 |
| Country |
Canada |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE132458 |
Effects of Peroxisome proliferator-γ coactivator-1α (PGC-1a) isoform over-expression +/- TNFalpha on hepatocyte gene expression |
|
