|
| Status |
Public on Apr 01, 2009 |
| Title |
infected 4 weeks |
| Sample type |
RNA |
| |
|
| Source name |
mouse liver
|
| Organism |
Mus musculus |
| Characteristics |
tissue: liver
gender: male
strain: FVB/N
|
| Treatment protocol |
The mice at 6 weeks of age were infected via oral inoculation with 15 metacercariae of C. sinensis in saline.
|
| Growth protocol |
The FVB/N male mice were maintained in a temperature-controlled (21 ± 1℃) and humidity-controlled (55 ± 5%) barrier system, with a 12 hr light/dark photocycle.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with BIO-RAD Experion.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion TotalPrep RNA Amplification Kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
n/a
|
| Data processing |
The data were normalised using rank invariant normalisation with BeadStudio
|
| |
|
| Submission date |
Mar 30, 2009 |
| Last update date |
Mar 31, 2009 |
| Contact name |
Suk-Jin Yang |
| Organization name |
Korea Research Institute of Bioscience & Biottechnology
|
| Department |
Medical Genomics Research Center
|
| Street address |
111 Gwahanggno, Yuseong-gu
|
| City |
Daejeon |
| ZIP/Postal code |
305-806 |
| Country |
South Korea |
| |
|
| Platform ID |
GPL6103 |
| Series (1) |
| GSE15447 |
Gene expression profiling in mouse liver infected with Clonorchis sinensis metacercariae. |
|
