|
| Status |
Public on Sep 01, 2009 |
| Title |
Ago2 knockout mouse mature oocyte#3 |
| Sample type |
RNA |
| |
|
| Source name |
Ago2 knockout mouse mature oocyte
|
| Organism |
Mus musculus |
| Characteristics |
genotype: Ago2 knockout
cell type: mature oocyte
replicate: 3
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to manufacturer's instructions
|
| Label |
digoxigenin
|
| Label protocol |
Digoxigenin labeled cRNA was prepared according to Applied Biosystems nanoamp RT-IVT protocol
|
| |
|
| Hybridization protocol |
Hybridization was performed according to Applied Biosystems Chemiluminescence Detection Kit Protocol (PN 4339627) using 10 ug fragmented cRNA
|
| Scan protocol |
Image was captured using CCD camera came with AB1700 Microarray Analyzer
|
| Description |
The Ago2 floxed mice were mated with Zp3-Cre transgenic mice to obtain [Ago2F/F, Zp3-Cre] conditional knockout females
Gene expression data from single cell (AGo2 knockout mouse oocyte) using Applied Biosystems AB1700 Whole Genome Microarray
|
| Data processing |
Quantile normalization was performed separately for knockout and wild-type samples. The normalization was performed using ABarray bioconductor package
|
| |
|
| Submission date |
Apr 02, 2009 |
| Last update date |
Apr 06, 2009 |
| Contact name |
Fuchou Tang |
| Organization name |
University of Cambridge
|
| Department |
Wellcome Trust/Cancer Research UK Gurdon Institute
|
| Street address |
Tennis Court Road
|
| City |
Cambridge |
| State/province |
Cambridgeshire |
| ZIP/Postal code |
CB2 1QN |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL2995 |
| Series (1) |
| GSE15529 |
Essential role for Argonaute2 protein in mouse oogenesis |
|
