|
Status |
Public on Oct 06, 2009 |
Title |
Sample_MIRG2 |
Sample type |
RNA |
|
|
Source name |
Sample_MIRG
|
Organism |
Homo sapiens |
Characteristics |
ETS2 and ERG promote megakaryopoiesis and synergize with alterations in GATA1 to immortalize hematopoietic progenitor cells Sample MIRG2
|
Extracted molecule |
total RNA |
Extraction protocol |
standard as recommended by illumina
|
Label |
Cy3
|
Label protocol |
standard as recommended by illumina
|
|
|
Hybridization protocol |
standard as recommended by illumina
|
Scan protocol |
standard as recommended by illumina
|
Description |
Serial replated GFP + lineage negative cells from Gata1neoHS fetal liver hematopoietic progenitors for 4 generations in methocult M3234 under megakaryocyte promoting conditions, IL3,TPO, IL6, and IL11. Cells were subject to retro-viral infection with MIGR1GFP, MIGR1ETS2, MIGR1ERG, and MIGR1FLI1. Used Qiagen Rneasy plus mini kit.
|
Data processing |
The data was preprocessed by Bioconductor lumi package (version 1.7.18). It was vst transformed and quantile normalized.
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|
|
Submission date |
Apr 02, 2009 |
Last update date |
Oct 05, 2009 |
Contact name |
monika stankiewicz |
Organization name |
Northwestern University
|
Department |
Hematology/Oncology
|
Lab |
Crispino
|
Street address |
303 E. Superior St. Lurie Bldg 5-250
|
City |
Chicago |
State/province |
IL |
ZIP/Postal code |
60611 |
Country |
USA |
|
|
Platform ID |
GPL6103 |
Series (1) |
GSE14506 |
ETS2, ERG promote megakaryopoiesis, synergize with alterations in GATA1 to immortalize hematopoietic progenitor cells |
|