|
| Status |
Public on Oct 06, 2009 |
| Title |
Sample_MIRG4 |
| Sample type |
RNA |
| |
|
| Source name |
Sample_MIRG
|
| Organism |
Homo sapiens |
| Characteristics |
ETS2 and ERG promote megakaryopoiesis and synergize with alterations in GATA1 to immortalize hematopoietic progenitor cells Sample MIRG4
|
| Extracted molecule |
total RNA |
| Extraction protocol |
standard as recommended by illumina
|
| Label |
Cy3
|
| Label protocol |
standard as recommended by illumina
|
| |
|
| Hybridization protocol |
standard as recommended by illumina
|
| Scan protocol |
standard as recommended by illumina
|
| Description |
Serial replated GFP + lineage negative cells from Gata1neoHS fetal liver hematopoietic progenitors for 4 generations in methocult M3234 under megakaryocyte promoting conditions, IL3,TPO, IL6, and IL11. Cells were subject to retro-viral infection with MIGR1GFP, MIGR1ETS2, MIGR1ERG, and MIGR1FLI1. Used Qiagen Rneasy plus mini kit.
|
| Data processing |
The data was preprocessed by Bioconductor lumi package (version 1.7.18). It was vst transformed and quantile normalized.
|
| |
|
| Submission date |
Apr 02, 2009 |
| Last update date |
Oct 05, 2009 |
| Contact name |
monika stankiewicz |
| Organization name |
Northwestern University
|
| Department |
Hematology/Oncology
|
| Lab |
Crispino
|
| Street address |
303 E. Superior St. Lurie Bldg 5-250
|
| City |
Chicago |
| State/province |
IL |
| ZIP/Postal code |
60611 |
| Country |
USA |
| |
|
| Platform ID |
GPL6103 |
| Series (1) |
| GSE14506 |
ETS2, ERG promote megakaryopoiesis, synergize with alterations in GATA1 to immortalize hematopoietic progenitor cells |
|
