|
| Status |
Public on Apr 04, 2009 |
| Title |
Endometrium, severe negative energy balance_UCIC11 |
| Sample type |
RNA |
| |
|
| Source name |
Endometrium from cow 11 severe negative energy balance
|
| Organism |
Bos taurus |
| Characteristics |
tissue: uterine endometrial tissue
genotype: Holstein-Friesian cow, black and white
age: 4.7 lactations
|
| Treatment protocol |
From day 2 after calving mild (MNEB) cows were fed ad lib grass silage with 8 kg/day of a 21% crude protein dairy concentrate and milked x1 daily; severe (SNEB) cows were fed 25 kg/day silage with 4 kg/day concentrate and milked x3 daily. The chemical composition of silage and concentrate offered (as previously described (53)) was the same across treatment groups. Daily measurements of milk yield, milk composition, dry matter intake (DMI), body weight, and dietary energy intake were used to calculate EB, based on the French net energy for lactation (NEL) system. Net EB was calculated as UFL/day in which 1 unité fourragère lait (UFL) is the NEL equivalent of 1 kg of standard air-dry barley as described previously (29). Samples of uterine endometrium were collected from all cows following slaughter at 14 ± 0.4 days postpartum.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Based on the established ARK-Genomics protocols (http://www.ark-genomics.org/protocols).
|
| |
|
| Hybridization protocol |
Based on the established ARK-Genomics protocols (http://www.ark-genomics.org/protocols).
|
| Scan protocol |
GeneChips were scanned using Affymetrix 3000 7G, Fluidics station.
|
| Description |
Gene expression data of endometrial tissue from severe negative energy balance cow
Multiparous Holstein-Friesian cows with a mean parity of 4.7 and an average previous lactation yield of 6,477 ± 354 kg were used. Cows were blocked two weeks prior to expected calving according to parity, body condition score and previous yield and randomly allocated to 2 treatments designed to produce mild (n=5) or severe NEB (n=6).
|
| Data processing |
CHP files were not used for data analysis. The CEL files were processed using S+ArrayAnalyzer 2.1 (Insightful Corporation, Seattle, Washington). The raw data were summarized using Robust Multichip Analysis (RMA) with a primary Quantile normalization. The summarized data were further normalized with a Median Inter-quartile Range (IQR).
|
| |
|
| Submission date |
Apr 03, 2009 |
| Last update date |
Apr 03, 2009 |
| Contact name |
Zhangrui Cheng |
| E-mail(s) |
zcheng@rvc.ac.uk
|
| Phone |
01707666663
|
| Organization name |
Royal Veterinary College
|
| Department |
Production and Population Health
|
| Lab |
Reproduction
|
| Street address |
Hawkshead Lane
|
| City |
Hatfield |
| State/province |
Hertfordshire |
| ZIP/Postal code |
AL9 7TA |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL2112 |
| Series (1) |
| GSE15544 |
Expression data from uterine endometrial tissues of Holstein-Friesian cows on days 14-16 after calving |
|
