|
| Status |
Public on Aug 31, 2019 |
| Title |
WT Wing imaginal disc 10x scRNA-seq |
| Sample type |
SRA |
| |
|
| Source name |
Imaginal wing disc
|
| Organism |
Drosophila melanogaster |
| Characteristics |
strain: BL:2035
tissue: Wing imaginal disc
age: 96 Hour After Egg Laying
genotype: FRT82B
|
| Growth protocol |
Around 50 embryos collected within 3 hours were put in an individual vial of fly food to avoid crowding and the larvae were raised at 25-degree incubator for appropriate lengths of time before dissection.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The wing imaginal discs were dissociated in 0.25% Trypsin-EDTA solution at 37 ℃ for 10 min. Cells were then washed in DPBS and passed through 35μm filter before library preparation.
Construction of 10x single cell libraries and sequencing on Illumina Hiseq platform were performed by Novogene.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Data processing |
Raw data mapping and primary analysis was performed in the Cell Ranger pipeline.
Secondary analysis for marker gene expression pattern was performed with Seurat (Butler et al., 2018).
Genome_build: GCF_000001215.4
Supplementary_files_format_and_content: csv files include normalized abundance measurements for each Sample
|
| |
|
| Submission date |
Jun 24, 2019 |
| Last update date |
Sep 01, 2019 |
| Contact name |
Yan Yan |
| E-mail(s) |
drosophilaust@gmail.com
|
| Organization name |
HKUST
|
| Department |
LIFS
|
| Street address |
Clear Water Bay
|
| City |
Hong Kong |
| ZIP/Postal code |
999077 |
| Country |
China |
| |
|
| Platform ID |
GPL25244 |
| Series (1) |
| GSE133204 |
Single-cell transcriptomic analysis of 96hour AEL wild type wing imaginal discs |
|
| Relations |
| BioSample |
SAMN12125918 |
| SRA |
SRX6354121 |
