|
| Status |
Public on Jun 27, 2019 |
| Title |
Wild type B6(Cg)-Tyrc-2J/J (p16+/+) mice |
| Sample type |
RNA |
| |
|
| Source name |
Jackson Mice
|
| Organism |
Mus musculus |
| Characteristics |
tissue: lung lysate
activation agent: control
time: 24 weeks old
genotype: WT
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted with Zymo RNA isolation Kit followed by DNase I treatment.
|
| Label |
SYBR Green
|
| Label protocol |
QIAGEN PAMM030 RT2 Profiler PCR Arrays were used to determine the genes altered in the insulin signaling pathway. Fast SYBR Green master max (ThermoFisher) was used during the reaction. Qiagen’s Data Analysis software was used to analyze results and construct a heatmap. All genes are normalized to 5 housekeeping genes within the array
|
| |
|
| Hybridization protocol |
n/a
|
| Scan protocol |
n/a
|
| Description |
Control
|
| Data processing |
Qiagen’s Data Analysis software was used to analyze results and construct a heatmap. Lysates were collected from 5 treated lungs (4 lungs for p16 KO room air group) and were averaged. All genes are normalized to 5 housekeeping genes within the array
For the normalization it uses the average of five housekeeping genes: B2M, HPRT1 , RPL13A, GAPDH, ACTB. Values from the different treated lungs were averaged within
Target gene signals normalized to housekeeping genes; 2^-deltaCt, where deltaCt = (Ct_Target − Ct_HKG)]
The web-based software package automatically performs all deltadeltaCt based fold-change calculations from the uploaded raw threshold cycle data.
Fold Change worksheet reports test/control (i.e.,Room air wt/ experimental CS) ratios.
|
| |
|
| Submission date |
Jun 26, 2019 |
| Last update date |
Jun 27, 2019 |
| Contact name |
Christopher Travis Cottage |
| E-mail(s) |
cottagec@medimmune.com
|
| Phone |
3013980007
|
| Organization name |
AstraZeneca
|
| Street address |
1 Medimmune Way
|
| City |
Gaithersburg |
| State/province |
MD |
| ZIP/Postal code |
20878 |
| Country |
USA |
| |
|
| Platform ID |
GPL26852 |
| Series (1) |
| GSE133380 |
Targeting p16-induced Senescence Prevents Cigarette Smoke-Induced Emphysema by Promoting IGF1/Akt1 Signaling in mice |
|
