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Sample GSM3907032 Query DataSets for GSM3907032
Status Public on Jun 27, 2019
Title p16Luc mice (Strain Code 01XBT -- B6.Cg-Cdkn2a tm3.1Nesh Tyr c-2J/Nci)
Sample type RNA
 
Source name Jackson Mice
Organism Mus musculus
Characteristics tissue: lung lysate
activation agent: p16(-/-) room air
time: 24 weeks old
genotype: p16 -/-
Extracted molecule total RNA
Extraction protocol Total RNA was extracted with Zymo RNA isolation Kit followed by DNase I treatment.
Label SYBR Green
Label protocol QIAGEN PAMM030 RT2 Profiler PCR Arrays were used to determine the genes altered in the insulin signaling pathway. Fast SYBR Green master max (ThermoFisher) was used during the reaction. Qiagen’s Data Analysis software was used to analyze results and construct a heatmap. All genes are normalized to 5 housekeeping genes within the array
 
Hybridization protocol n/a
Scan protocol n/a
Description Test
Data processing Qiagen’s Data Analysis software was used to analyze results and construct a heatmap. Lysates were collected from 5 treated lungs (4 lungs for p16 KO room air group) and were averaged. All genes are normalized to 5 housekeeping genes within the array
For the normalization it uses the average of five housekeeping genes: B2M, HPRT1 , RPL13A, GAPDH, ACTB. Values from the different treated lungs were averaged within
Target gene signals normalized to housekeeping genes; 2^-deltaCt, where deltaCt = (Ct_Target − Ct_HKG)]
The web-based software package automatically performs all deltadeltaCt based fold-change calculations from the uploaded raw threshold cycle data.
Fold Change worksheet reports test/control (i.e.,Room air wt/ experimental CS) ratios.
 
Submission date Jun 26, 2019
Last update date Jun 27, 2019
Contact name Christopher Travis Cottage
E-mail(s) cottagec@medimmune.com
Phone 3013980007
Organization name AstraZeneca
Street address 1 Medimmune Way
City Gaithersburg
State/province MD
ZIP/Postal code 20878
Country USA
 
Platform ID GPL26852
Series (1)
GSE133380 Targeting p16-induced Senescence Prevents Cigarette Smoke-Induced Emphysema by Promoting IGF1/Akt1 Signaling in mice

Data table header descriptions
ID_REF
VALUE normalized signal (against housekeeping genes).

Data table
ID_REF VALUE
A01 0.271829
A02 0.178305
A03 0.202835
A04 0.186083
A05 0.135076
A06 0.211292
A07 0.00244
A08 0.04617
A09 0.044284
A10 0.031871
A11 0.02738
A12 0.022916
B01 0.116374
B02 0.096626
B03 0.045296
B04 0.12934
B05 0.125904
B06 0.423216
B07 0.148468
B08 0.171564

Total number of rows: 89

Table truncated, full table size 1 Kbytes.




Supplementary data files not provided
Processed data included within Sample table
Processed data are available on Series record
Raw data are available on Series record

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