|
| Status |
Public on Jun 19, 2009 |
| Title |
M1 MR+ |
| Sample type |
RNA |
| |
|
| Source name |
bone marrow derived DC
|
| Organism |
Mus musculus |
| Characteristics |
cell type: dendritic cells (DCs)
mannose receptor: positive
|
| Treatment protocol |
Immediately after FACS sort, bone marrow derived DC were lysed in Trizol reagent
|
| Growth protocol |
Freshly isolated bone marrow derived cells were differentiated for 1 week with GM-CSF into bone marrow derived DCs. Afterwards, cells were incubated with Alexa647-labeled OVA to distinguish mannose receptor positive from mannose receptor negative cells by FACS analysis.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with NanoDrop ThermoScientific.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
replicate 2
|
| Data processing |
The data were normalised using quantile normalisation in R
|
| |
|
| Submission date |
Apr 09, 2009 |
| Last update date |
Dec 19, 2012 |
| Contact name |
Joachim Schultze |
| E-mail(s) |
j.schultze@uni-bonn.de
|
| Organization name |
LIMES (Life and Medical Sciences Center Genomics and Immunoregulation)
|
| Department |
Genomics and Immunoregulation
|
| Street address |
Carl-Troll-Strasse 31
|
| City |
Bonn |
| State/province |
NRW |
| ZIP/Postal code |
53115 |
| Country |
Germany |
| |
|
| Platform ID |
GPL6887 |
| Series (1) |
| GSE15616 |
Increased antigen cross-presentation but impaired cross-priming after activation of PPARĪ³ |
|
