|
| Status |
Public on Dec 16, 2010 |
| Title |
15-LOX2 splice variant b transgenic (svb9) vs wild type (wt)_VP_R2 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
wild type (2.5 month old) mice ventral prostate
|
| Organism |
Mus musculus |
| Characteristics |
tissue: ventral prostate
age: 2.5 month old
genetic background: FVB
genotype: wild type
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from combined ventral prostates of three 2.5 month old wild type mice after mincing with Biopulverizer (Biospec products) and using an RNAeasy RNA purification kit (Qiagen, Valencia, CA), including on-column DNase digestion to completely remove contaminating genomic DNA. RNA concentrations were determined in NanoDrop and the quality assessed using a Bioanalyzer.
|
| Label |
Cy-3
|
| Label protocol |
labeling was done according to manufacturer's protocol available at http://www.chem.agilent.com/temp/rad71A57/00001389.PDF
|
| |
|
| Channel 2 |
| Source name |
15-LOX2 splice variant b transgenic (svb9, 2.5 month old) mice ventral prostate
|
| Organism |
Mus musculus |
| Characteristics |
tissue: ventral prostate
genotype: 15-Lipoxygenase 2 (15-LOX2) splice variant b (sv-b) transgenic mice (line svb9),
age: 2.5 month old
genetic background: FVB
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from combined ventral prostates of three 2.5 month old svb9 mice after mincing with Biopulverizer (Biospec products) and using an RNAeasy RNA purification kit (Qiagen, Valencia, CA), including on-column DNase digestion to completely remove contaminating genomic DNA. RNA concentrations were determined in NanoDrop and the quality assessed using a Bioanalyzer.
|
| Label |
Cy-5
|
| Label protocol |
labeling was done according to manufacturer's protocol available at http://www.chem.agilent.com/temp/rad71A57/00001389.PDF
|
| |
|
| |
| Hybridization protocol |
Manufacturer's protocol available at http://www.chem.agilent.com/temp/rad71A57/00001389.PDF
|
| Scan protocol |
Scanning and data acquisition were performed using Agilent scanner and feature extraction software version A.7.5.1 (www.agilent.com).
|
| Description |
The current array analysis was carried out to study the changes in the gene expression profile of 2.5 month old 15-LOX2 splice variant b (sv-b) transgenic mice (line svb9) ventral prostate (VP) compared to age matched wild type mice VP.
|
| Data processing |
The data was acquired using Agilent's feature extraction software (version A.7.5.1) with inbuilt Lowess normalization feature and Logratio algorithms, for details visit www.agilent.com
|
| |
|
| Submission date |
Apr 17, 2009 |
| Last update date |
Dec 16, 2010 |
| Contact name |
Dean G Tang |
| E-mail(s) |
dtang@mdanderson.org
|
| Organization name |
U.T M. D. Anderson Cancer Center
|
| Department |
Carcinogenesis
|
| Lab |
Dean G Tang's Lab
|
| Street address |
1808 Park road 1C
|
| City |
Smithville |
| State/province |
TX |
| ZIP/Postal code |
78957 |
| Country |
USA |
| |
|
| Platform ID |
GPL2872 |
| Series (1) |
| GSE15827 |
Gene expression analysis of 15-Lipoxygenase 2 (15-LOX2) transgenic mice |
|
