|
| Status |
Public on Sep 15, 2021 |
| Title |
HBE-Al2O3 NPs [R21] |
| Sample type |
RNA |
| |
|
| Source name |
HBE,100 μg/ml Al2O3,24h
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HBE
treatment: Al2O3 NPs
|
| Treatment protocol |
Cells were exposed to 100 µg/ml Al2O3 NPs，100/500 µg/ml PM2.5, 50 µg/mlDEP.
|
| Growth protocol |
The HBE cells (American Type Culture Collection) were maintained in Dulbecco′s modified Eagle′s medium (DMEM). The culture medium was supplemented with 10 % (v/v) fetal bovine serum (FBS), 100 U/ml penicillin, and 100 μg/ml streptomycin at 37 °C with 5% CO2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using TRIzol (Invitrogen) and miRNeasy mini kit (QIAGEN) according to manufacturer’s instructions, which efficiently recovered all RNA species, including miRNAs. RNA quality and quantity was measured by using nanodrop spectrophotometer (ND-1000, Nanodrop Technologies) and RNA Integrity was determined by gel electrophoresis
|
| Label |
Hy3
|
| Label protocol |
The miRCURY™ Hy3™/Hy5™ Power labeling kit (Exiqon, Vedbaek, Denmark) was used according to the manufacturer’s guideline for miRNA labelling
|
| |
|
| Hybridization protocol |
The Hy3TM-labeled samples were hybridized on the miRCURYTM LNA Array (v.16.0) (Exiqon) according to array manual. Full array name: Exiqon miRCURY LNA microRNA array, 6th generation [miRBase v16]
|
| Scan protocol |
The slides were scanned using the Axon GenePix 4000B microarray scanner (Axon Instruments, Foster City, CA).
|
| Description |
The HBE cells were available from American Type Culture Collection)
|
| Data processing |
Scanned images were then imported into GenePix Pro 6.0 software (Axon) for grid alignment and data extraction. Replicated miRNAs were averaged and miRNAs that intensities>=50 in all samples were chosen for calculating normalization factor. Expressed data were normalized using the Median normalization. After normalization, differentially expressed miRNAs with statistical significance were identified through Volcano Plot filtering
Processed data file includes Normalized signal intensity with probe ID and miRNA symbol.
|
| |
|
| Submission date |
Jul 17, 2019 |
| Last update date |
Sep 15, 2021 |
| Contact name |
Rui Chen |
| Organization name |
Capital medical university
|
| Street address |
No.10 Xitoutiao, You An Men
|
| City |
Beijing |
| ZIP/Postal code |
100069 |
| Country |
China |
| |
|
| Platform ID |
GPL17107 |
| Series (1) |
| GSE134430 |
MicroRNA microarray of HBE cell exposed to Al2O3 NPs, PM2.5, DEP |
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