|
Status |
Public on Oct 11, 2019 |
Title |
DU145 radioresistant vs. Normal |
Sample type |
genomic |
|
|
Channel 1 |
Source name |
Radioresistant DU145
|
Organism |
Homo sapiens |
Characteristics |
cell line: derived from DU145 phenotype: radioresistant Sex: Male
|
Treatment protocol |
Radioresistant sub-line was generated from original (parental) DU145 or LNCaP cell line by multiple X-ray irradiations, 4Gy/dose, 1 week recovery between doses. Prior to RNA isolation cells were stained with Aldefluor kit (Stem Cell Technologies) and sorted by FACS.
|
Growth protocol |
Prostate cancer cell lines DU145 and LNCaP were purchased from the American Type Culture Collection (ATCC, Manassas, VA) and cultured according to the manufacturers recommendations in a humidified 37°C incubator supplemented with 5% CO2. DU145 cells were maintained in Dulbecco's Modified Eagle's Medium (DMEM) (Sigma-Aldrich) and LNCaP cells in RPMI-1640 medium (Sigma-Aldrich) containing 10% fetal bovine serum (FBS, PAA Laboratories) and 1mM L-glutamine (Sigma-Aldrich).
|
Extracted molecule |
genomic DNA |
Extraction protocol |
DNA was extracted from cell cultures and normal tumor tissue by phenol:chloroform extraction using standard protocols.
|
Label |
Cy3
|
Label protocol |
Labeling was done according to the manufacturer‘s instructions with the exception that the labeling of reference and test DNA was reversed.
|
|
|
Channel 2 |
Source name |
Agilent Euro Male
|
Organism |
Homo sapiens |
Characteristics |
tissue: Normal tissue Sex: Male
|
Treatment protocol |
Radioresistant sub-line was generated from original (parental) DU145 or LNCaP cell line by multiple X-ray irradiations, 4Gy/dose, 1 week recovery between doses. Prior to RNA isolation cells were stained with Aldefluor kit (Stem Cell Technologies) and sorted by FACS.
|
Growth protocol |
Prostate cancer cell lines DU145 and LNCaP were purchased from the American Type Culture Collection (ATCC, Manassas, VA) and cultured according to the manufacturers recommendations in a humidified 37°C incubator supplemented with 5% CO2. DU145 cells were maintained in Dulbecco's Modified Eagle's Medium (DMEM) (Sigma-Aldrich) and LNCaP cells in RPMI-1640 medium (Sigma-Aldrich) containing 10% fetal bovine serum (FBS, PAA Laboratories) and 1mM L-glutamine (Sigma-Aldrich).
|
Extracted molecule |
genomic DNA |
Extraction protocol |
DNA was extracted from cell cultures and normal tumor tissue by phenol:chloroform extraction using standard protocols.
|
Label |
Cy5
|
Label protocol |
Labeling was done according to the manufacturer‘s instructions with the exception that the labeling of reference and test DNA was reversed.
|
|
|
|
Hybridization protocol |
Hybridization was done according to the manufacturer‘s instructions.
|
Scan protocol |
Arrays were scanned using the Agilent Technologies Scanner G2505C according to the manufacturer‘s instructions.
|
Description |
aCGH profiling DU145_RR
|
Data processing |
Feature extraction and processing of raw data were done with Agilent’s CytoGenomics Editions 2.7 (DU145) and 2.9 (LNCaP).
|
|
|
Submission date |
Jul 18, 2019 |
Last update date |
Oct 11, 2019 |
Contact name |
Michael Seifert |
Organization name |
TU Dresden
|
Street address |
Fetscherstr. 74
|
City |
Dresden |
ZIP/Postal code |
01307 |
Country |
Germany |
|
|
Platform ID |
GPL11357 |
Series (2) |
GSE134498 |
DNA copy number alteration analysis of radioresistant and radiosensitive prostate cancer cell lines |
GSE134500 |
DNA copy number alteration analysis and gene expression profiles comparing radioresistant to radiosensitive prostate cancer cell lines |
|