|
| Status |
Public on Oct 11, 2019 |
| Title |
DU145 radiosensitive vs. Normal |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
Radiosensitive DU145
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: parental DU145
phenotype: radiosensitive
Sex: Male
|
| Treatment protocol |
Radioresistant sub-line was generated from original (parental) DU145 or LNCaP cell line by multiple X-ray irradiations, 4Gy/dose, 1 week recovery between doses. Prior to RNA isolation cells were stained with Aldefluor kit (Stem Cell Technologies) and sorted by FACS.
|
| Growth protocol |
Prostate cancer cell lines DU145 and LNCaP were purchased from the American Type Culture Collection (ATCC, Manassas, VA) and cultured according to the manufacturers recommendations in a humidified 37°C incubator supplemented with 5% CO2. DU145 cells were maintained in Dulbecco's Modified Eagle's Medium (DMEM) (Sigma-Aldrich) and LNCaP cells in RPMI-1640 medium (Sigma-Aldrich) containing 10% fetal bovine serum (FBS, PAA Laboratories) and 1mM L-glutamine (Sigma-Aldrich).
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
DNA was extracted from cell cultures and normal tumor tissue by phenol:chloroform extraction using standard protocols.
|
| Label |
Cy3
|
| Label protocol |
Labeling was done according to the manufacturer‘s instructions with the exception that the labeling of reference and test DNA was reversed.
|
| |
|
| Channel 2 |
| Source name |
Agilent Euro Male
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Normal tissue
Sex: Male
|
| Treatment protocol |
Radioresistant sub-line was generated from original (parental) DU145 or LNCaP cell line by multiple X-ray irradiations, 4Gy/dose, 1 week recovery between doses. Prior to RNA isolation cells were stained with Aldefluor kit (Stem Cell Technologies) and sorted by FACS.
|
| Growth protocol |
Prostate cancer cell lines DU145 and LNCaP were purchased from the American Type Culture Collection (ATCC, Manassas, VA) and cultured according to the manufacturers recommendations in a humidified 37°C incubator supplemented with 5% CO2. DU145 cells were maintained in Dulbecco's Modified Eagle's Medium (DMEM) (Sigma-Aldrich) and LNCaP cells in RPMI-1640 medium (Sigma-Aldrich) containing 10% fetal bovine serum (FBS, PAA Laboratories) and 1mM L-glutamine (Sigma-Aldrich).
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
DNA was extracted from cell cultures and normal tumor tissue by phenol:chloroform extraction using standard protocols.
|
| Label |
Cy5
|
| Label protocol |
Labeling was done according to the manufacturer‘s instructions with the exception that the labeling of reference and test DNA was reversed.
|
| |
|
| |
| Hybridization protocol |
Hybridization was done according to the manufacturer‘s instructions.
|
| Scan protocol |
Arrays were scanned using the Agilent Technologies Scanner G2505C according to the manufacturer‘s instructions.
|
| Description |
aCGH profiling
DU145_RS
|
| Data processing |
Feature extraction and processing of raw data were done with Agilent’s CytoGenomics Editions 2.7 (DU145) and 2.9 (LNCaP).
|
| |
|
| Submission date |
Jul 18, 2019 |
| Last update date |
Oct 11, 2019 |
| Contact name |
Michael Seifert |
| Organization name |
TU Dresden
|
| Street address |
Fetscherstr. 74
|
| City |
Dresden |
| ZIP/Postal code |
01307 |
| Country |
Germany |
| |
|
| Platform ID |
GPL11357 |
| Series (2) |
| GSE134498 |
DNA copy number alteration analysis of radioresistant and radiosensitive prostate cancer cell lines |
| GSE134500 |
DNA copy number alteration analysis and gene expression profiles comparing radioresistant to radiosensitive prostate cancer cell lines |
|
