|
| Status |
Public on Apr 16, 2010 |
| Title |
podocyte, control 2 |
| Sample type |
RNA |
| |
|
| Source name |
podocyte, control
|
| Organism |
Mus musculus |
| Characteristics |
cell: podocyte
|
| Treatment protocol |
untreated
|
| Growth protocol |
the cell line was maintained in RPMI 1640supplemented with 10% fetal bovine serum, 100 U/ml penicillin/streptomycin in humidified incubators with air-5% CO2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA.
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
Affymetrix GeneArray Scanner3000
|
| Description |
Gene expression data from control podocytes
|
| Data processing |
The data were median-polished and quantile normalized with JMP Genomics Software.
|
| |
|
| Submission date |
Apr 21, 2009 |
| Last update date |
Apr 22, 2009 |
| Contact name |
Li Li |
| E-mail(s) |
li.li@gmx.de
|
| Organization name |
University Heidelberg
|
| Street address |
Theodor-Kutzer-Ufer
|
| City |
Mannheim |
| ZIP/Postal code |
68167 |
| Country |
Germany |
| |
|
| Platform ID |
GPL8462 |
| Series (1) |
|
