|
| Status |
Public on Apr 14, 2010 |
| Title |
NIC_I_2 |
| Sample type |
RNA |
| |
|
| Source name |
Striatum of 6-10 week old C57BL/6J mice
|
| Organism |
Mus musculus |
| Characteristics |
timepoint: 1h
drug: Nicotine
group no.: 32
large batch: Y
plate: 4164379045
hybridization batch: IV
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
Replicate 2
|
| Data processing |
The data were normalised using quantile normalisation with IlluminaGUI in R
|
| |
|
| Submission date |
Apr 22, 2009 |
| Last update date |
May 02, 2009 |
| Contact name |
Marcin Piechota |
| E-mail(s) |
marpiech@if-pan.krakow.pl
|
| Phone |
(+4812) 6623328
|
| Organization name |
Institute of Pharmacology PAS
|
| Department |
Molecular Neuropharmacology
|
| Street address |
Smetna 12
|
| City |
Krakow |
| ZIP/Postal code |
31-343 |
| Country |
Poland |
| |
|
| Platform ID |
GPL6105 |
| Series (1) |
| GSE15774 |
Transcriptional networks regulated by drugs of abuse in mouse striatum |
|
