|
| Status |
Public on Apr 24, 2009 |
| Title |
Formoterol 4H Rep 1 |
| Sample type |
RNA |
| |
|
| Source name |
tibialis anterior
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/10 ScSn
treatment: Formoterol
treatment time: 4 Hours
|
| Treatment protocol |
Mice were randomly assigned and injected with a single intraperitoneal injection of either formoterol (100 μg/kg in 0.2mL saline) or saline. Following treatment for 1 or 4 hours, mice were anesthetized and tibialis anterior muscles were surgically excised for RNA extraction.
|
| Growth protocol |
Male C57BL/10 ScSn (wild type, 6-7 weeks old) mice were housed in the Biological Research Facility at The University of Melbourne and maintained on a 12 h-light/12 h-dark cycle, with standard mouse chow and water provided ad libitum.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with tri-reagent (Sigma), followed by treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser 2100.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Illumina TotalPrep RNA Amplification kit (Ambion)
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Scanned with Illumina BeadStation Scanner.
|
| Description |
1512403075_D
Replicate 1
|
| Data processing |
Data was compiled using BeadStudio v1.5.1.3 (Illumina). Normalized data was produced using GeneSpring GX version 7.3.1 via normalization to control genes, where control genes were represented by all genes with an Illumina detection score equal to one in at least four out of the 16 samples (7596 control genes in total).
|
| |
|
| Submission date |
Apr 23, 2009 |
| Last update date |
Apr 23, 2009 |
| Contact name |
Michael Andrew Pearen |
| E-mail(s) |
m.pearen@uq.edu.au
|
| Phone |
+61 7 33462365
|
| Fax |
+61 7 33462101
|
| Organization name |
Institute for Molecular Bioscience
|
| Lab |
Group Muscat
|
| Street address |
The University of Queensland
|
| City |
St Lucia |
| State/province |
Queensland |
| ZIP/Postal code |
4072 |
| Country |
Australia |
| |
|
| Platform ID |
GPL6333 |
| Series (1) |
| GSE15793 |
Expression profiling of skeletal muscle following acute β2-adrenergic stimulation |
|
