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Sample GSM396536 Query DataSets for GSM396536
Status Public on Jan 19, 2011
Title Muscle_NormalFood_Swimming
Sample type RNA
 
Source name Muscle, 14days, normal food, swimming
Organism Mus musculus
Characteristics gender: male
age: Six-week-old
tissue: swimming muscle
Extracted molecule total RNA
Extraction protocol A part of the muscle tissue was then placed in RNAlater RNA Stabilization Reagent (Qiagen, Inc., Valencia, CA, USA) and was kept overnight at 4°C and subsequently stored at 20°C. RNA was isolated using an RNeasy Mini Kit (Qiagen) and was purified according to the manufacturer’s instructions. RNA purity and concentration were evaluated with a spectrophotometer using an absorbance ratio of 260/280 nm.
Label Cy3
Label protocol The total RNA of each group was pooled (n = 10 for each group) and labeled with Cyanine 3 (Perkin Elmer, Boston, MA, USA) using 500 ng of total RNA, according to the manufacturer’s instructions, which generated one-color labeled cRNAs.
 
Hybridization protocol We used One-Color Microarray-Based Gene Expression Analysis (Quick Amp Labeling) v5.5 and according to the manufacturer’s instructions.
Scan protocol Microarray was scanned by DNA Microarray Scanner G2565BA (Agilent Technologies, Palo Alto, CA, USA).
Description Gene expression after 14days in swimming mouse muscle
Data processing The intensities were measured using Agilent Feature Extraction Software 9.5 (Agilent Technologies). A global normalization was performed for the inter-chip normalization. In order to define the expression level of the gene/probe, the signal intensity of each gene on a chip was divided by the median value of the signal intensities of the entire genes on the chip.
 
Submission date Apr 23, 2009
Last update date Jan 19, 2011
Contact name Toru Mizoguchi
E-mail(s) mizoguchi@sunchlorella.co.jp
Phone +81-75-288-3010
Fax +81-75-288-3011
URL http://www.sunchlorella.com/
Organization name Sun Chlorella Corp.
Department Research and Development Department
Street address 369, Karasuma-dori, Gojo, Shimogyo-ku
City Kyoto
State/province Kyoto
ZIP/Postal code 600-8177
Country Japan
 
Platform ID GPL4134
Series (1)
GSE15798 Regulation of the anti-immune response by Chlorella intake during swimming stress in mice

Data table header descriptions
ID_REF
VALUE A global normalization was performed for the inter-chip normalization. In order to define the expression level of the gene/probe, the signal intensity of each gene on a chip was divided by the median value of the signal intensities of the entire genes on the chip.

Data table
ID_REF VALUE
4669 -5.764685787
23234 0.701676402
42403 -0.958304785
32933 -0.577668266
33750 -0.345969704
34803 0.01967114
27433 -0.940593164
23820 -0.421731471
14382 0.114526038
1668 0.599813996
38972 0.031067959
7833 0.23434968
13494 -0.005437404
28169 -2.160264432
35126 0.073549581
11591 1.411221437
27127 -8.60E-08
24649 -1.027584646
14476 -1.078567454
6188 1.849545141

Total number of rows: 13683

Table truncated, full table size 243 Kbytes.




Supplementary file Size Download File type/resource
GSM396536.txt.gz 7.8 Mb (ftp)(http) TXT
Processed data included within Sample table

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