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Sample GSM399373 Query DataSets for GSM399373
Status Public on May 02, 2009
Title T.4SP24int.Th#1
Sample type RNA
 
Source name thymus
Organism Mus musculus
Characteristics age: 6 weeks
exclusion markers: 11b- 11c- 19- 49b- Gr1- TER119-
genetic background: C57BL/6J
phenotype markers: 4+ 8- TCRhi 24int
separation method: dissection/trimming
Sex: male
Extracted molecule total RNA
Extraction protocol Glass slide teasing Flow sorting
Label Biotin
Label protocol Affy GeneChip WT cDNA Synthesis kit/cRNA Cleanup kit
 
Hybridization protocol Affy 1.0 ST Hyb Kit
Scan protocol Affymetrix GeneChip Scanner
Description T.4SP24int.Th#1, CD4 Single-Positive, Semimature
Data processing The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
 
Submission date Apr 30, 2009
Last update date May 24, 2016
Contact name Liang Yang
Organization name Harvard Medical School
Department Pathology
Lab CBDM
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platform ID GPL6246
Series (1)
GSE15907 ImmGen Microarray Phase 1

Data table header descriptions
ID_REF
VALUE RMA normalized signal intensity

Data table
ID_REF VALUE
10344620 20.0844
10344622 282.553
10344624 428.0177
10344633 694.1966
10344637 205.187
10344653 19.2136
10344658 148.2845
10344674 11.0243
10344679 45.4864
10344707 180.9955
10344713 371.2692
10344723 263.4504
10344725 42.4765
10344741 2927.5114
10344743 26.9051
10344750 485.6386
10344772 27.2561
10344789 135.0214
10344797 319.8811
10344799 158.9883

Total number of rows: 24922

Table truncated, full table size 422 Kbytes.




Supplementary file Size Download File type/resource
GSM399373_EA07068_52777_MoGene_T.4SP24int.Th_#1.cel.gz 4.1 Mb (ftp)(http) CEL
Processed data included within Sample table

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