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Sample GSM399382 Query DataSets for GSM399382
Status Public on May 02, 2009
Title T.8SP24int.Th#1
Sample type RNA
 
Source name thymus
Organism Mus musculus
Characteristics age: 6 weeks
exclusion markers: 11b- 11c- 19- 49b- Gr1- TER119-
genetic background: C57BL/6J
phenotype markers: 4- 8+ TCRhi 24int
separation method: dissection/trimming
Sex: male
Extracted molecule total RNA
Extraction protocol Glass slide teasing Flow sorting
Label Biotin
Label protocol Affy GeneChip WT cDNA Synthesis kit/cRNA Cleanup kit
 
Hybridization protocol Affy 1.0 ST Hyb Kit
Scan protocol Affymetrix GeneChip Scanner
Description T.8SP24int.Th#1, CD8 Single-Positive, Semimature
Data processing The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
 
Submission date Apr 30, 2009
Last update date May 24, 2016
Contact name Liang Yang
Organization name Harvard Medical School
Department Pathology
Lab CBDM
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platform ID GPL6246
Series (1)
GSE15907 ImmGen Microarray Phase 1

Data table header descriptions
ID_REF
VALUE RMA normalized signal intensity

Data table
ID_REF VALUE
10344620 16.7627
10344622 285.4284
10344624 395.2614
10344633 742.3832
10344637 212.191
10344653 16.6724
10344658 218.1484
10344674 10.2463
10344679 43.0981
10344707 259.5207
10344713 323.4651
10344723 228.8164
10344725 39.597
10344741 3369.0638
10344743 28.3086
10344750 570.6222
10344772 24.6956
10344789 209.3708
10344797 321.6059
10344799 204.7547

Total number of rows: 24922

Table truncated, full table size 422 Kbytes.




Supplementary file Size Download File type/resource
GSM399382_EA07068_52783_MoGene_T.8SP24int.Th_#1.cel.gz 3.8 Mb (ftp)(http) CEL
Processed data included within Sample table

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