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Sample GSM399395 Query DataSets for GSM399395
Status Public on May 02, 2009
Title T.DP69+.Th#2
Sample type RNA
 
Source name thymus
Organism Mus musculus
Characteristics age: 6 weeks
exclusion markers: 11b- 11c- 19- 49b- Gr1- TER119-
genetic background: C57BL/6J
phenotype markers: 4+ 8+ TCRlo/int 69+
separation method: dissection/trimming
Sex: male
Extracted molecule total RNA
Extraction protocol Glass slide teasing Flow sorting
Label Biotin
Label protocol Affy GeneChip WT cDNA Synthesis kit/cRNA Cleanup kit
 
Hybridization protocol Affy 1.0 ST Hyb Kit
Scan protocol Affymetrix GeneChip Scanner
Description T.DP69+.Th#2, Double-Positive, Early Positive Selection
Data processing The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
 
Submission date Apr 30, 2009
Last update date May 24, 2016
Contact name Liang Yang
Organization name Harvard Medical School
Department Pathology
Lab CBDM
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platform ID GPL6246
Series (1)
GSE15907 ImmGen Microarray Phase 1

Data table header descriptions
ID_REF
VALUE RMA normalized signal intensity

Data table
ID_REF VALUE
10344620 18.6085
10344622 253.5422
10344624 384.127
10344633 497.5341
10344637 196.7962
10344653 25.5671
10344658 132.0715
10344674 11.5995
10344679 43.189
10344707 162.8325
10344713 284.0347
10344723 266.7145
10344725 37.1267
10344741 2365.0015
10344743 29.4846
10344750 314.3056
10344772 30.7807
10344789 133.0891
10344797 401.622
10344799 165.6123

Total number of rows: 24922

Table truncated, full table size 422 Kbytes.




Supplementary file Size Download File type/resource
GSM399395_EA07068_52766_MoGene_T.DP69+.Th_#2.cel.gz 3.7 Mb (ftp)(http) CEL
Processed data included within Sample table

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