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Sample GSM399439 Query DataSets for GSM399439
Status Public on May 02, 2009
Title B.FrE.BM#3
Sample type RNA
 
Source name Bone marrow
Organism Mus musculus
Characteristics age: 8 weeks
exclusion markers: CD3 Ly6c Ter119 Gr1 Mac1
genetic background: C57BL/6J
phenotype markers: CD19+ IgM+ AA4.1+ HSA+
separation method: dissection/trimming
Sex: male
Extracted molecule total RNA
Extraction protocol disruption by 1ml syringe, ACK lysis of RBCs
Label Biotin
Label protocol Affy GeneChip WT cDNA Synthesis kit/cRNA Cleanup kit
 
Hybridization protocol Affy 1.0 ST Hyb Kit
Scan protocol Affymetrix GeneChip Scanner
Description B.FrE.BM#3, Fr. E (newly-formed B)
Data processing The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
 
Submission date Apr 30, 2009
Last update date May 24, 2016
Contact name Liang Yang
Organization name Harvard Medical School
Department Pathology
Lab CBDM
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platform ID GPL6246
Series (1)
GSE15907 ImmGen Microarray Phase 1

Data table header descriptions
ID_REF
VALUE RMA normalized signal intensity

Data table
ID_REF VALUE
10344620 15.733
10344622 267.0236
10344624 337.1521
10344633 770.0799
10344637 216.8116
10344653 12.0665
10344658 249.8557
10344674 11.3217
10344679 32.2714
10344707 294.7982
10344713 306.1753
10344723 232.0199
10344725 38.1293
10344741 2778.7565
10344743 29.2547
10344750 469.0262
10344772 32.2548
10344789 232.7685
10344797 342.251
10344799 295.2338

Total number of rows: 24922

Table truncated, full table size 422 Kbytes.




Supplementary file Size Download File type/resource
GSM399439_EA07068_56613_MoGene_B.FrE.BM_#3.cel.gz 4.1 Mb (ftp)(http) CEL
Processed data included within Sample table

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