NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM399453 Query DataSets for GSM399453
Status Public on May 02, 2009
Title preB.FrC.BM#3
Sample type RNA
 
Source name Bone marrow
Organism Mus musculus
Characteristics age: 8 weeks
exclusion markers: CD3 Ly6c Ter119 Gr1 Mac1
genetic background: C57BL/6J
phenotype markers: CD19+ IgM- CD45R+ CD43+ HSA+
separation method: dissection/trimming
Sex: male
Extracted molecule total RNA
Extraction protocol disruption by 1ml syringe, ACK lysis of RBCs
Label Biotin
Label protocol Affy GeneChip WT cDNA Synthesis kit/cRNA Cleanup kit
 
Hybridization protocol Affy 1.0 ST Hyb Kit
Scan protocol Affymetrix GeneChip Scanner
Description preB.FrC.BM#3, Fr. Cprime (cycling pre-B)
Data processing The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
 
Submission date Apr 30, 2009
Last update date May 24, 2016
Contact name Liang Yang
Organization name Harvard Medical School
Department Pathology
Lab CBDM
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platform ID GPL6246
Series (1)
GSE15907 ImmGen Microarray Phase 1

Data table header descriptions
ID_REF
VALUE RMA normalized signal intensity

Data table
ID_REF VALUE
10344620 15.6276
10344622 402.5289
10344624 555.5049
10344633 906.1934
10344637 239.9338
10344653 13.2816
10344658 230.0659
10344674 8.6716
10344679 35.2255
10344707 391.6452
10344713 1427.9716
10344723 390.5758
10344725 35.1803
10344741 5395.8963
10344743 23.7164
10344750 223.193
10344772 24.5962
10344789 351.8458
10344797 234.3762
10344799 380.7584

Total number of rows: 24922

Table truncated, full table size 422 Kbytes.




Supplementary file Size Download File type/resource
GSM399453_EA07068_56611_MoGene_preB.FrC.BM_#3.cel.gz 4.7 Mb (ftp)(http) CEL
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap