|
| Status |
Public on Feb 08, 2012 |
| Title |
15M-HighFat 18% |
| Sample type |
RNA |
| |
|
| Source name |
Liver
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57bl/6J OlaHsd
gender: Male
age: 20wks
tissue: Liver
diet: HighFat 18%
|
| Treatment protocol |
Pregnant females were fed a control diet (18%) or a low protein diet 9% (90 g casein/kg) during the gestational period, offspring was placed after 6 wks on HF diet (36% SF) or a control diet for 14 wks.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with TriReagent (Sigma). Quality control was performed with Agilent Bioanalyzer.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Illumina TotalPrep RNA Amplification Kit (Applied Biosystems)
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
Biological replicate 3
|
| Data processing |
The data after background substraction were normalized using quantile normalization with BeadStudio Expression Module 3.2.7
|
| |
|
| Submission date |
May 04, 2009 |
| Last update date |
Feb 09, 2012 |
| Contact name |
vincent bloks |
| Organization name |
UMCG
|
| Department |
Pediatrics
|
| Lab |
Pediatrics
|
| Street address |
Hanzeplein1
|
| City |
Groningen |
| ZIP/Postal code |
9713GZ |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL6103 |
| Series (1) |
| GSE15940 |
Sex-Dependent Programming of Glucose and Fatty Acid Metabolism in Mouse Offspring by Maternal Protein Restriction |
|
