Strain: C57BL6 Heart: atria, Female total RNA (MoHe.FeAt) received from National Human Genome Research Institute was analyzed through Bioanalyzer and passed our quality control test. The mRNA was processed according to the MPSS protocol as outlined in the previous publications (Brenner, S., et al. (2000) Proc Natl Acad Sci U S A 97(4): 1665-1670 and Brenner, S. et al., Nat. Biotechnol 18(6): 630-634). Briefly, the mRNA was reverse transcribed and the cDNA was digested with Dpn II. The 20 bases adjacent to the 3? most Dpn II site was cloned into a Megaclone vector. The resulting library was amplified and loaded onto microbeads. About 1.6 million microbeads were loaded into each flow cell and the signature sequences were determined by a series of enzymatic reactions as outlined in the above publications. The abundance for each signature was converted to transcripts per million (tpm) for the purpose of comparisons between samples. Cells/tissue: Library MoHe.FeAt.sig21 Cell type Heart: atria, Female Source NHGRI RNA isolation LYNX mRNA QC passed cDNA library: Library DpnII restriction - (signature cloning using MmeI) Sequence length 20 bp MPSS: runs MoHe.FeAt_sig21.5311W.b-20 1/18/2005 405582 2242 QC Passed MoHe.FeAt_sig21.5311F.b-20 1/18/2005 229484 3445 QC Passed MoHe.FeAt_sig21.5311W.d-20 1/17/2005 458621 3982 QC Passed MoHe.FeAt_sig21.5311F.c-20 1/15/2005 367322 4596 QC Passed MoHe.FeAt_sig21.5311F.a-20 12/17/2004 297373 3404 QC Passed MoHe.FeAt_sig21.5141F.c-20 10/18/2004 293934 3339 QC Passed MoHe.FeAt_sig21.5141W.b-20 8/18/2004 492639 3144 QC Passed Run group: Total Beads successfully sequenced - 2544955 Processed Signatures - 6970