Strain: C57BL6 Lung, Female total RNA (MoLu.Feml) received from National Human Genome Research Institute was analyzed through Bioanalyzer and passed our quality control test. The mRNA was processed according to the MPSS protocol as outlined in the previous publications (Brenner, S., et al. (2000) Proc Natl Acad Sci U S A 97(4): 1665-1670 and Brenner, S. et al., Nat. Biotechnol 18(6): 630-634). Briefly, the mRNA was reverse transcribed and the cDNA was digested with Dpn II. The 20 bases adjacent to the 3? most Dpn II site was cloned into a Megaclone vector. The resulting library was amplified and loaded onto microbeads. About 1.6 million microbeads were loaded into each flow cell and the signature sequences were determined by a series of enzymatic reactions as outlined in the above publications. The abundance for each signature was converted to transcripts per million (tpm) for the purpose of comparisons between samples. Cells/tissue: Library MoLu.Feml.sig21 Cell type Lung, Female Source NHGRI RNA isolation LYNX mRNA QC passed cDNA library: Library DpnII restriction - (signature cloning using MmeI) Sequence length 20 bp MPSS: runs MoLu.Feml_sig21.5286W.d-20 1/11/2005 289684 9496 QC Passed MoLu.Feml_sig21.5286W.b-20 12/18/2004 268693 6630 QC Passed MoLu.Feml_sig21.5286F.a-20 12/6/2004 430604 10702 QC Passed MoLu.Feml_sig21.5286F.b-20 12/6/2004 491316 10063 QC Passed MoLu.Feml_sig21.5149F.a-20 10/12/2004 394448 10130 QC Passed MoLu.Feml_sig21.5149W.a-20 8/30/2004 581725 13890 QC Passed Run group: Total Beads successfully sequenced - 2456470 Processed Signatures - 19159