|
| Status |
Public on May 12, 2010 |
| Title |
foxo4 knock out mice two days after treatment of 50% ethanol enema |
| Sample type |
RNA |
| |
|
| Source name |
colon mucosa
|
| Organism |
Mus musculus |
| Characteristics |
strain: FVB/N6
age: 8 weeks
genetic modification: foxo4 knock out
tissue: Colon Mucosa
treatment: 50% ethanol enema
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyzer.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
replicate 4
|
| Data processing |
The data were normalized using quantile normalization with IlluminaGUI in R
|
| |
|
| Submission date |
May 08, 2009 |
| Last update date |
May 12, 2009 |
| Contact name |
wen zhou |
| E-mail(s) |
wen.zhou@utsouthwestern.edu
|
| Phone |
214-648-4024
|
| Organization name |
UT Southwestern Medical Center at Dallas
|
| Department |
Internal Medicine ,Cardiology
|
| Lab |
Liu Lab
|
| Street address |
6000 Harry Hines Blvd NB11.110
|
| City |
Dallas |
| State/province |
TX |
| ZIP/Postal code |
75235 |
| Country |
USA |
| |
|
| Platform ID |
GPL6105 |
| Series (1) |
| GSE16033 |
Microarray analysis of colonic mucosa gene expression in wild type and foxo4 knock out mice |
|
