|
Status |
Public on May 12, 2009 |
Title |
MIG_tp 2_biological replicate 2 |
Sample type |
RNA |
|
|
Channel 1 |
Source name |
CD34+ control cells, 3 days post transduction, replicate 2
|
Organism |
Homo sapiens |
Characteristics |
cell type: CD34+ cord blood cells time: 3 days post transduction sample type: control
|
Biomaterial provider |
Umbilical cord blood samples were collected from normal deliveries after obtaining informed consent. Retroviral vectors were provided as described (Järås et al., 2009, Exp Hematol)
|
Treatment protocol |
none
|
Growth protocol |
cells were grown as described (Järås et al., 2009, Exp Hematol)
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction followed by purification using RNeasy
|
Label |
Cy3
|
Label protocol |
Samples were labeled using the Agilent low RNA input fluorescent linear amplification kit (Agilent Technologies)
|
|
|
Channel 2 |
Source name |
Stratagene Universal Human Reference RNA
|
Organism |
Homo sapiens |
Characteristics |
sample type: RNA pooled from ten human cell lines
|
Biomaterial provider |
Stratagene
|
Treatment protocol |
none
|
Growth protocol |
none
|
Extracted molecule |
total RNA |
Extraction protocol |
None
|
Label |
Cy5
|
Label protocol |
Samples were labeled using the Agilent low RNA input fluorescent linear amplification kit (Agilent Technologies)
|
|
|
|
Hybridization protocol |
Arrays were hybridized and washed as described (Järås et al., 20089, Exp Hematol)
|
Scan protocol |
Arrays were scanned in an Agilent Microarray scanner
|
Description |
oligo microarray data
|
Data processing |
Spot quality was analyzed with GenePix 4.0 software. Raw data were normalized (pin-based Lowess regression) and filtered (SNR > 2, SD >= 0.2, presence >= 90%) in the BioArray Software Environment (BASE).
|
|
|
Submission date |
May 11, 2009 |
Last update date |
May 11, 2009 |
Contact name |
Petra Johnels |
E-mail(s) |
petra.johnels@med.lu.se, thoas.fioretos@med.lu.se
|
Phone |
+46 46 173398
|
Fax |
+46 46 131061
|
Organization name |
Lund University
|
Department |
Clinical Genetics
|
Street address |
Lund University Hospital
|
City |
Lund |
ZIP/Postal code |
221 85 |
Country |
Sweden |
|
|
Platform ID |
GPL5886 |
Series (1) |
GSE16042 |
Retroviral transduction of CD34+ cells with P190 and P210 BCR/ABL1 fusion genes |
|