|
| Status |
Public on Jul 21, 2009 |
| Title |
Prostate_obstruction_Eviprostat_rep2 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
obstruction, Eviprostat
|
| Organism |
Rattus norvegicus |
| Characteristics |
gender: Male
age: 10 week
tissue: Prostate
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Sepasol-RNA I (Nacalai Tesque) following manufacturer's protocol.
|
| Label |
Cy5
|
| Label protocol |
Total RNA from the pooled prostate tissue from group 1 (1500 ng) and groups 2-4 (300 ng each) was reverse transcribed into cDNA with MMLV-RT and oligo dT-promoter primer, and the resulting cDNA was used for the synthesis of fluorescently labeled cRNA sample probes with the Agilent Quick Amp Labeling Kit, following manufacturer's protocol.
|
| |
|
| Channel 2 |
| Source name |
sham, vehicle
|
| Organism |
Rattus norvegicus |
| Characteristics |
gender: Male
age: 10 week
tissue: Prostate
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Sepasol-RNA I (Nacalai Tesque) following manufacturer's protocol.
|
| Label |
Cy3
|
| Label protocol |
Total RNA from the pooled prostate tissue from group 1 (1500 ng) and groups 2-4 (300 ng each) was reverse transcribed into cDNA with MMLV-RT and oligo dT-promoter primer, and the resulting cDNA was used for the synthesis of fluorescently labeled cRNA sample probes with the Agilent Quick Amp Labeling Kit, following manufacturer's protocol.
|
| |
|
| |
| Hybridization protocol |
Blocking Agent and Fragmentation Buffer (Agilent Gene Expression Hybridization kit) were added to the samples, and incubated at 60 C for 30min. GE Hybridization Buffer HI-RPM was added, and samples were applied to microarrays enclosed in Agilent 4x44K gasket slides. Slides were incubated at 65 C for 1 day, and washed sequential with Agilent Gene Expression Wash Pack, following manufacturer's protocol.
|
| Scan protocol |
Scanned on an Agilent G2505A scanner.
Images were quantified using Agilent Feature Extraction Software (v 9.5.1.1).
|
| Description |
Biological replicate 2 of 3. Partial bladder outlet obstruction. Treated twice a day with 18 mg/kg Eviprostat for five days.
|
| Data processing |
Agilent Feature Extraction Software (v 9.5.1.1) was used for background subtraction and LOWESS normalization.
|
| |
|
| Submission date |
May 12, 2009 |
| Last update date |
Jul 21, 2009 |
| Contact name |
Mitsuhiro Tagaya |
| E-mail(s) |
m.tagaya@nippon-shinyaku.co.jp
|
| Phone |
81-29-850-6242
|
| Fax |
81-29-850-6217
|
| Organization name |
Nippon Shinyaku co.,ltd.
|
| Department |
Discovery Research Laboratories
|
| Street address |
3-14-1 Sakura
|
| City |
Tsukuba |
| State/province |
Ibaraki |
| ZIP/Postal code |
305-0003 |
| Country |
Japan |
| |
|
| Platform ID |
GPL4135 |
| Series (1) |
| GSE16063 |
Prostates of rats with induced partial bladder outlet obstruction and the effect of Eviprostat on those changes |
|
