|
Status |
Public on May 16, 2009 |
Title |
PKGI KO vs. WT brown adipose tissues replicate 2 |
Sample type |
RNA |
|
|
Channel 1 |
Source name |
WT
|
Organism |
Mus musculus |
Characteristics |
tissue: brown adipose genotype: WT genetic background: C57BL/6N
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol
|
Label |
Cy3
|
Label protocol |
Agilent Low RNA Input Linear Amp Kit
|
|
|
Channel 2 |
Source name |
PKGI KO
|
Organism |
Mus musculus |
Characteristics |
tissue: brown adipose genotype: PKGI KO genetic background: C57BL/6N
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol
|
Label |
Cy5
|
Label protocol |
Agilent Low RNA Input Linear Amp Kit
|
|
|
|
Hybridization protocol |
Agilent Gene Expression Hybridization Kit
|
Scan protocol |
DNA microarray scanner (Agilent)
|
Description |
all steps according to manufacturer's recommendations
|
Data processing |
Agilent Feature Extraction Software default lowess normalization
|
|
|
Submission date |
May 14, 2009 |
Last update date |
May 15, 2009 |
Contact name |
Peter Mayer |
E-mail(s) |
pmayer@bfarm.de
|
Organization name |
Fed. Inst. for Drugs and Med. Devices
|
Street address |
Kurt-Georg-Kiesinger-Allee
|
City |
Bonn |
ZIP/Postal code |
53111 |
Country |
Germany |
|
|
Platform ID |
GPL4134 |
Series (1) |
GSE16106 |
PKGI KO vs. WT brown adipocytes and brown adipose tissues |
|
Data table header descriptions |
ID_REF |
|
VALUE |
UNF_VALUE: normalized log ratio (rProcessedSignal/gProcessedSignal) base 10 w/ flags removed |
PositionX |
|
PositionY |
|
LogRatioError |
|
PValueLogRatio |
|
gSurrogateUsed |
|
rSurrogateUsed |
|
gIsFound |
signal intensity green (measured) |
rIsFound |
signal intensity red (measured) |
gProcessedSignal |
signal intensity green (processed) |
rProcessedSignal |
signal intensity red (processed) |
gProcessedSigError |
|
rProcessedSigError |
|
gNumPixOLHi |
|
rNumPixOLHi |
|
gNumPixOLLo |
|
rNumPixOLLo |
|
gNumPix |
|
rNumPix |
|
gMeanSignal |
|
rMeanSignal |
|
gMedianSignal |
|
rMedianSignal |
|
gPixSDev |
|
rPixSDev |
|
gPixNormIQR |
|
rPixNormIQR |
|
gBGNumPix |
|
rBGNumPix |
|
gBGMeanSignal |
|
rBGMeanSignal |
|
gBGMedianSignal |
|
rBGMedianSignal |
|
gBGPixSDev |
|
rBGPixSDev |
|
gBGPixNormIQR |
|
rBGPixNormIQR |
|
gNumSatPix |
|
rNumSatPix |
|
gIsSaturated |
|
rIsSaturated |
|
gIsLowPMTScaledUp |
|
rIsLowPMTScaledUp |
|
PixCorrelation |
|
BGPixCorrelation |
|
gIsFeatNonUnifOL |
|
rIsFeatNonUnifOL |
|
gIsBGNonUnifOL |
|
rIsBGNonUnifOL |
|
gIsFeatPopnOL |
|
rIsFeatPopnOL |
|
gIsBGPopnOL |
|
rIsBGPopnOL |
|
IsManualFlag |
|
gBGSubSignal |
|
rBGSubSignal |
|
gBGSubSigError |
|
rBGSubSigError |
|
BGSubSigCorrelation |
|
gIsPosAndSignif |
|
rIsPosAndSignif |
|
gPValFeatEqBG |
|
rPValFeatEqBG |
|
gNumBGUsed |
|
rNumBGUsed |
|
gIsWellAboveBG |
|
rIsWellAboveBG |
|
gBGUsed |
|
rBGUsed |
|
gBGSDUsed |
|
rBGSDUsed |
|
IsNormalization |
|
gDyeNormSignal |
|
rDyeNormSignal |
|
gDyeNormError |
|
rDyeNormError |
|
DyeNormCorrelation |
|
ErrorModel |
|
xDev |
|
gSpatialDetrendIsInFilteredSet |
|
rSpatialDetrendIsInFilteredSet |
|
gSpatialDetrendSurfaceValue |
|
rSpatialDetrendSurfaceValue |
|
SpotExtentX |
|
SpotExtentY |
|
gNetSignal |
|
rNetSignal |
|
gMultDetrendSignal |
|
rMultDetrendSignal |
|
gProcessedBackground |
|
rProcessedBackground |
|
gProcessedBkngError |
|
rProcessedBkngError |
|
IsUsedBGAdjust |
|
gInterpolatedNegCtrlSub |
|
rInterpolatedNegCtrlSub |
|
gIsInNegCtrlRange |
|
rIsInNegCtrlRange |
|
gIsUsedInMD |
|
rIsUsedInMD |
|
UNF_VALUE |
normalized log ratio (rProcessedSignal/gProcessedSignal) base 10 |