|
| Status |
Public on May 21, 2009 |
| Title |
Neurospheres in selection N1 |
| Sample type |
RNA |
| |
|
| Source name |
366.4 cells
|
| Organism |
Macaca mulatta |
| Characteristics |
developmental stage: N1
cell line: 366.4
|
| Growth protocol |
The rhesus monkey embryonic cells derived from flushed preimplantation embryohas been characterize for its pluripotency including its potential differentiation to neural stages. All reagents in the cell culture experiments were used from Invitrogen corporation. ESC cell from passage 13 were collected as (ESC) and propagated on MEF layer with DMEM Eagle's media.(ESC stage). after 6-8 days 1-1.5 mm embroid bodies were collected as (EB). EBs werew then cultured in DMEM/F12 mudium with N2 supplement for 7-9days.After 9days 0..25-1.25 mm speres of N1 collected(N1stage). N1 further grow in the medium MDEM/F12/N2 suppliment and added FGF4 for expansion of Nestin positive cell and collected after 7-9days(N2 stage). The neurospheres of stage N2 further extended in neuronal maturation phase with in GFR reduced well in DMEM/F12 medium without FGF4.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA extraction with Qiagen RNAeasy microkit
|
| Label |
Biotin
|
| Label protocol |
Biotinylated Enzo labelling of RNA
|
| |
|
| Hybridization protocol |
Affymetrix standard protocol
|
| Scan protocol |
Affymetrix standard protocol
|
| Description |
rhesus stem cell
|
| Data processing |
GCOS 1.0
|
| |
|
| Submission date |
May 20, 2009 |
| Last update date |
May 20, 2009 |
| Contact name |
Arubala P. Reddy |
| E-mail(s) |
reddya@ohsu.edu
|
| Phone |
503 690 5330
|
| Fax |
504 690 5384
|
| Organization name |
Oregon Health and Sciences University
|
| Department |
Oregon National Primate Research Center
|
| Lab |
Reproductive sciences
|
| Street address |
505 NW 185th Ave
|
| City |
Beaverton |
| State/province |
OR |
| ZIP/Postal code |
97006 |
| Country |
USA |
| |
|
| Platform ID |
GPL3535 |
| Series (1) |
| GSE16168 |
Expression Profile of Embryonic Stem Cell Derived Serotonin Neurons |
|
