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Sample GSM406311 Query DataSets for GSM406311
Status Public on Jul 29, 2009
Title ESC rep1
Sample type RNA
Source name Embryonic Stem Cells
Organism Mus musculus
Characteristics cell type: Embryonic Stem Cells
potency: Pluripotent
Extracted molecule total RNA
Extraction protocol RNA samples to be analyzed on microarrays were prepared using Qiagen RNeasy columns with on-column DNA digestion.
Label biotin
Label protocol 400 ng of total DNA-free RNA was used as input for cRNA synthesis (Illumina TotalPrep RNA Amplification Kit - Ambion) following the manufacturer's instructions (IVT: 12h).
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
Hybridization protocol Standard Illumina hybridization protocol. Quality-checked cRNA samples were hybridized as biological or technical duplicates for 18 h onto mouse-8 v2 expression BeadChips (Illumina), washed, and stained following guidelines and using materials / instrumentation supplied / suggested by the manufacturer.
Scan protocol Standard Illumina scanning protocol. Scanned following guidelines and using materials / instrumentation supplied / suggested by the manufacturer.
Description replicate 1
Data processing The bead intensities were mapped to gene information using BeadStudio 3.2 (Illumina), background correction was perform using the Affymetrix Robust Multi-array Analysis (RMA) background correction model, variance stabilization was performed using the log2 scaling and gene expression normalization was calculated with the quantile method implemented in the lumi package of R-Bioconductor.
Submission date May 20, 2009
Last update date Jul 29, 2009
Contact name Marcos J Ara├║zo-Bravo
Organization name Max Planck Institute for Molecular Biomedicine
Department Cell and Developmental Biology
Lab Computational Biology and Bioinformatics
Street address Rontgenstr. 20
City Muenster
ZIP/Postal code 48149
Country Germany
Platform ID GPL6885
Series (1)
GSE16178 Induction of Pluripotency in Adult Unipotent Germline Stem Cells

Data table header descriptions
VALUE log2, quantile-normalized signal intensities

Data table
ILMN_1212607 1.330486
ILMN_1212612 2.043674
ILMN_1212619 2.411736
ILMN_1212628 1.664374
ILMN_1212632 6.081083
ILMN_1212636 11.726976
ILMN_1212637 7.880883
ILMN_1212645 1.993609
ILMN_1212648 7.466449
ILMN_1212653 2.887248
ILMN_1212672 7.724944
ILMN_1212682 3.600071
ILMN_1212683 0.922447
ILMN_1212685 1.860782
ILMN_1212692 6.567058
ILMN_1212693 3.08684
ILMN_1212695 1.451346
ILMN_1212698 1.344508
ILMN_1212702 1.443442
ILMN_1212703 4.625658

Total number of rows: 25697

Table truncated, full table size 550 Kbytes.

Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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