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Sample GSM40723 Query DataSets for GSM40723
Status Public on Apr 20, 2005
Title Control 7-9-02
Sample type RNA
 
Source name Yeast BY4730
Organism Saccharomyces cerevisiae
Extracted molecule total RNA
 
Description BY4730 cultures were exposed to medium containing dilutions of test agent (Cisplatin (Cis), 600mg/mL; Methylmethane sulfonate (MMS), 0.12% v/v; Bleomycin (Bleo), 0.15U/mL; NaCl, 10mg/mL; ethanol, 15% v/v) for 120 min. Control cultures were either mock-treated with the solvent, DMSO (for comparison to Cis, MMS, Bleo) or maintained in media (for comparison to ethanol and NaCl) for 120 mins. Sample preparation and processing procedures were performed according the Affymetrix Genechipâ Expression Analysis Technical Manual (Affymetrix, Santa Clara, CA). Briefly, total RNA was isolated from thawed cell pellets using a hot phenol protocol described by Schmitt et. al. (1990). Total RNA was converted to poly(A)+ mRNA and isolated using Oligotex mRNA kit. Double stranded cDNA was generated by using T7-(dT) 24 oligo primer (Genset Corp., San Diego, CA) and Superscript Double Stranded cDNA Synthesis Kit (Gibco BRL, Carlsbad, CA). The cDNA was used for in vitro transcription (IVT) with Enzo BioArray RNA Transcript Labeling Kit to incorporate biotinylated ribonucleotides. Biotinylated cRNA (15 mg) was fragmented in 40 mM tris-acetate, pH 8.1; 30 mM MgOAc; 100 mM KOAc. Samples were then hybridized with Affymetrix probe array Yeast Genome S98 at 45°C for 16 hours with constant rotation. Hybridized probe arrays were washed and stained using an Affymetrix GeneChipâ Fluidics Station 400 and protocol EukGE-WS2. The chips were scanned using the Affymetrix GeneArray Scanner.
 
Submission date Feb 01, 2005
Last update date Oct 28, 2005
Contact name Gregory R. Warnes
E-mail(s) gregory.r.warnes@pfizer.com
Phone 860-715-3536
Organization name Pfizer Inc., Groton, CT - USA
Lab Non-clinical Statistics
Street address Eastern Point Road
City Groton
State/province CT
ZIP/Postal code 06340
Country USA
 
Platform ID GPL90
Series (1)
GSE2224 Experimental condition

Data table header descriptions
ID_REF
VALUE RMA

Data table
ID_REF VALUE
10000_at 2.30601019472562
10001_at 5.2958885577522
10002_i_at 10.5964041535741
10003_f_at 11.5326360510235
10004_at 3.20405374415481
10005_at 7.00294492088701
10006_at 6.35603136714767
10007_at 5.16350667228156
10008_at 5.2725876923388
10009_at 5.07883145366089
10010_at 6.60713393099353
10011_at 2.44468525464391
10012_at 5.18162739526467
10013_at 4.84860811973502
10014_at 4.7276818612382
10015_at 6.26952214200493
10016_at 8.274281962188
10017_at 4.42996119801627
10018_at 3.66828892605458
10019_at 7.64718238832698

Total number of rows: 9335

Table truncated, full table size 232 Kbytes.




Supplementary data files not provided

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