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Sample GSM412776 Query DataSets for GSM412776
Status Public on Jan 19, 2010
Title R1E Mock 2
Sample type RNA
 
Source name R1E cells, mock
Organism Mus musculus
Characteristics cell line: R1E
agent: mock
Treatment protocol R1E cells were treated with 0.5 micromolar adriamycin or 25J/m2 UV for 8 hours in growth medium.
Growth protocol R1E cells were grown in mESC growth medium, Knockout DMEM (KO-DMEM), 15% Fetal Bovine Serum, 100mM MEM non-essential amino acids, 0.1mM 2-mercaptoethanol, 2mM L-glutamine and 1X penicillin-streptomycin (All from Invitrogen) plus 1000 units/ml leukemia inhibitory factor (Lif, Millipore)
Extracted molecule total RNA
Extraction protocol RNAs were extracted using the RNeasy mini kit (Qiagen).
Label biotin
Label protocol Affymetrix Whole Transcript Sense Target Labeling kit per manufacturer’s protocol.
 
Hybridization protocol Samples were hybridized to the Affymetrix Mouse Gene ST 1.0 GeneChip.
Scan protocol Arrays were scanned on the Affymetrix GeneChip scanner 3000.
Description R1E cells mock treated.
Data processing Data were processed with Affymetrix Expression Console (version 1.1). ANOVA, p-value <0.01; fold change>1.5. Transcripts that are significantly altered in both adriamycin and Ultraviolet treatments are shown. The subsequent statistical analyses were performed using R programming language version 2.8.1.

Probe group file: MoGene-1_0-st-v1.r4.pgf
Meta-probeset file: MoGene-1_0-st-v1.r4.mps
 
Submission date Jun 04, 2009
Last update date Dec 29, 2010
Contact name Jing Huang
E-mail(s) huangj3@mail.nih.gov
Organization name National Cancer Institute
Lab Cancer Biology and Genetics
Street address 37 Convent Dr. 37/3140
City Bethesda
State/province MD
ZIP/Postal code MD 20892
Country USA
 
Platform ID GPL6246
Series (3)
GSE16428 p53 regulates the Wnt signaling pathway in murine embryonic stem cells
GSE26360 Genome-wide analysis revealed a crosstalk between p53 and the pluripotent gene networks in mouse embryonic stem cells (expression)
GSE26362 Whole-genome study reveals distinct mechanisms used by p53 to regulate activated and repressed genes in embryonic stem cells

Data table header descriptions
ID_REF
VALUE RMA and quantile normalized signal intensity

Data table
ID_REF VALUE
10344614 7.630877
10344616 2.471149
10344618 2.607873
10344620 3.175356
10344622 5.996461
10344624 9.32339
10344633 9.659988
10344637 8.351117
10344653 3.818244
10344658 6.598173
10344674 2.91749
10344679 4.074146
10344705 3.179964
10344707 7.581213
10344713 11.40388
10344715 3.359371
10344717 3.293553
10344719 4.125768
10344721 2.340953
10344723 7.950941

Total number of rows: 28815

Table truncated, full table size 503 Kbytes.




Supplementary file Size Download File type/resource
GSM412776.CEL.gz 4.1 Mb (ftp)(http) CEL
Processed data included within Sample table

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