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| Status |
Public on Jan 01, 2021 |
| Title |
veh_rep2 |
| Sample type |
SRA |
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| Source name |
rat
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| Organism |
Rattus norvegicus |
| Characteristics |
strain: SD
tissue: brain
age: DIV 18
genotype: wild type
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| Extracted molecule |
polyA RNA |
| Extraction protocol |
Total RNA was extracted and purified using RNAiso Plus (TaKaRa)
RNA libraries were prepared according to instructions of Vazyme VAHTS mRNA-seq V3 Library Prep Kit for Illumina(NR611-01)
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
HiSeq X Ten |
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| Data processing |
For RNA-Seq comparison, Sequencing reads were mapped to rat with STAR by default parameter. The read counts for each genes were calculated by featurecounts. The count files were used as input to DESeq2 for differential expression analysis.
Genome_build: Rnor_6.0
Supplementary_files_format_and_content: DESeq was used for calling differential expression.tab-delimited text files include normalized read counts,foldChange,pvalue
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| Submission date |
Oct 18, 2019 |
| Last update date |
Jan 02, 2021 |
| Contact name |
jing zhao |
| E-mail(s) |
zhaojing@sioc.ac.cn
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| Phone |
18817665278
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| Organization name |
IRCBC
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| Street address |
Qiuyue Road
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| City |
shanghai |
| ZIP/Postal code |
20000 |
| Country |
China |
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| Platform ID |
GPL24688 |
| Series (1) |
| GSE139080 |
NMDA receptor positive allosteric modulator induced genome wide gene expression changes in neuronal cultures |
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| Relations |
| BioSample |
SAMN13054607 |
| SRA |
SRX7018723 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSM4130690_veh_rep2.txt.gz |
2.2 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
| Processed data are available on Series record |
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