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Sample GSM413500 Query DataSets for GSM413500
Status Public on Aug 13, 2009
Title E14 clone with pSicoR-mCherry Chd1 shRNAi1 C1i5
Sample type RNA
 
Source name ES cells, Chd1-deficient
Organism Mus musculus
Characteristics cell line: E14 clone C1i5
Treatment protocol Cells trypsinized with 0.05% Trypsin for 3min at 37C and resuspended in PBS with trypsin inactivator.
Growth protocol Cells on gelatin-coated plates with ES cell medium with 15% KSR and recombinant LIF.
Extracted molecule total RNA
Extraction protocol RNeasy extraction of total RNA was performed according to the manufacturer's instructions (Qiagen).
Label Biotin
Label protocol According to manufacturer's instructions at the Genomics Core Facility of the Gladstones Institutes.
 
Hybridization protocol According to manufacturer's instructions at the Genomics Core Facility of the Gladstones Institutes.
Scan protocol According to manufacturer's instructions at the Genomics Core Facility of the Gladstones Institutes.
Description Gene expression data of ES cells grown in normal ES cell conditions.
Data processing RMA
 
Submission date Jun 04, 2009
Last update date Aug 10, 2009
Contact name Miguel Ramalho-Santos
E-mail(s) mrsantos@diabetes.ucsf.edu
Organization name UCSF
Department Eli and Edythe Broad Center of Regeneration Medicine
Lab Santos lab
Street address 35 Medical Center Way
City San Francisco
State/province CA
ZIP/Postal code 94143
Country USA
 
Platform ID GPL6246
Series (1)
GSE16462 Expression data from Chd1-deficient mouse ES cells (E14 cell lines) and genome-wide binding of Chd1 in parental ES cells

Data table header descriptions
ID_REF
VALUE RMA values

Data table
ID_REF VALUE
10338001 12.50741
10338002 7.631042
10338003 11.15147
10338004 10.239
10338005 2.397471
10338006 2.899895
10338007 3.60886
10338008 5.101256
10338009 9.813913
10338010 2.512594
10338011 6.953693
10338012 2.626396
10338013 2.168679
10338014 2.234721
10338015 2.225952
10338016 9.210354
10338017 13.6347
10338018 8.250345
10338019 6.470732
10338020 9.842428

Total number of rows: 35557

Table truncated, full table size 621 Kbytes.




Supplementary file Size Download File type/resource
GSM413500.CEL.gz 4.3 Mb (ftp)(http) CEL
Processed data included within Sample table

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