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Sample GSM414316 Query DataSets for GSM414316
Status Public on Jul 01, 2009
Title Circumvallate taste buds, bottom portion, sample 3
Sample type RNA
 
Source name Bottom portion of CV taste buds
Organism Macaca fascicularis
Characteristics animal id number: 00834F
gender: Female
age (years): 3
tissue: Bottom portion of Circumvallate taste buds
Extracted molecule total RNA
Extraction protocol Extraction of total RNA was performed using a Qiagen microRNeasy kit according to the manufacturer's instructions.
Label biotin
Label protocol Total RNA was amplified to generate cDNA using the NuGEN Ovation Biotin RNA Amplification (v1). Labelled cDNA was prepared according to NuGEN Ovation Biotin RNA Amplification and Labeling System kit (v1). Material was labeled with Biotin.
 
Hybridization protocol Following fragmentation, approximately 2.0 ug of single stranded cDNA were hybridized for 18-20 hr at 45C on Affymetrix GeneChip Rhesus Macaque Genome Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 using the EukGE-WS2v4_450.
Scan protocol GeneChips were scanned using GeneChip® Scanner 3000 with Autoloader.
Description Material collected from ~5,000 cells by laser capture microdissection
Data processing The data were analyzed with Agilent Technologies GeneSpring GX software. Raw data were processed using GC-RMA normalization.
 
Submission date Jun 08, 2009
Last update date Jun 08, 2009
Contact name Bryan Moyer
E-mail(s) bmoyer@senomyx.com
Phone (858) 646 8327
Organization name Senomyx, Inc.
Street address 4767 Nexus Centre Drive
City San Diego
State/province CA
ZIP/Postal code 92121
Country USA
 
Platform ID GPL3535
Series (1)
GSE16485 Expression data from macaque taste buds and lingual epithelium

Data table header descriptions
ID_REF
VALUE GC-RMA signal intensity

Data table
ID_REF VALUE
MmugDNA.18507.1.S1_at 3.870390142
MmugDNA.23490.1.S1_at 5.472879151
MmugDNA.22851.1.S1_at 18.47548075
MmuSTS.1555.1.S1_at 1321.270371
MmugDNA.36797.1.S1_at 112.8744697
MmugDNA.29065.1.S1_at 13.11843759
MmugDNA.26408.1.S1_at 21.26356661
MmugDNA.1260.1.S1_at 107.8017658
MmugDNA.29982.1.S1_at 18.49449497
MmugDNA.17891.1.S1_at 393.9642748
MmugDNA.6758.1.S1_at 4.105653002
MmugDNA.42487.1.S1_at 4.292700738
MmugDNA.7889.1.S1_at 12.71282014
MmugDNA.25855.1.S1_at 3.010597206
MmugDNA.26543.1.S1_at 78.17571478
MmugDNA.16184.1.S1_at 27.11274914
MmugDNA.22894.1.S1_at 155.4689105
MmugDNA.3654.1.S1_at 13.11881314
MmugDNA.22796.1.S1_at 83.54043786
MmugDNA.40531.1.A1_at 11.8623357

Total number of rows: 52024

Table truncated, full table size 1697 Kbytes.




Supplementary file Size Download File type/resource
GSM414316.cel.gz 4.7 Mb (ftp)(http) CEL
Processed data included within Sample table

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