|
| Status |
Public on Jul 01, 2009 |
| Title |
Circumvallate taste buds, sample 3 |
| Sample type |
RNA |
| |
|
| Source name |
CV taste buds
|
| Organism |
Macaca fascicularis |
| Characteristics |
animal id number: 22419M
gender: Male
age (years): 3.8
tissue: Circumvallate taste buds
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Extraction of total RNA was performed using a Qiagen microRNeasy kit according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Total RNA was amplified to generate cDNA using the NuGEN Ovation Biotin RNA Amplification (v1). Labelled cDNA was prepared according to NuGEN Ovation Biotin RNA Amplification and Labeling System kit (v1). Material was labeled with Biotin.
|
| |
|
| Hybridization protocol |
Following fragmentation, approximately 2.0 ug of single stranded cDNA were hybridized for 18-20 hr at 45C on Affymetrix GeneChip Rhesus Macaque Genome Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 using the EukGE-WS2v4_450.
|
| Scan protocol |
GeneChips were scanned using GeneChip® Scanner 3000 with Autoloader.
|
| Description |
Material collected from ~5,000 cells by laser capture microdissection
|
| Data processing |
The data were analyzed with Agilent Technologies GeneSpring GX software. Raw data were processed using GC-RMA normalization.
|
| |
|
| Submission date |
Jun 08, 2009 |
| Last update date |
Jun 08, 2009 |
| Contact name |
Bryan Moyer |
| E-mail(s) |
bmoyer@senomyx.com
|
| Phone |
(858) 646 8327
|
| Organization name |
Senomyx, Inc.
|
| Street address |
4767 Nexus Centre Drive
|
| City |
San Diego |
| State/province |
CA |
| ZIP/Postal code |
92121 |
| Country |
USA |
| |
|
| Platform ID |
GPL3535 |
| Series (1) |
| GSE16485 |
Expression data from macaque taste buds and lingual epithelium |
|
