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Sample GSM414743 Query DataSets for GSM414743
Status Public on Jun 04, 2010
Title control_littermates_WT1
Sample type RNA
 
Source name control_littermates_WT
Organism Mus musculus
Characteristics tissue: Sciatic Nerve
gender: female and male
age: Post natal day 14
genotype: control littermates
Extracted molecule total RNA
Extraction protocol Tissue was collected from control littermates
We used the miRVana miRNA isolation kit from AmbionĀ®. We used the protocol to extract total RNA. Briefly, fresh frozen sciatic nerves were homogenization in lysis buffer followed by a phenol/chloroform extraction. The sample was then combined with 1.25 volumes of 100% ethanol, and passed through a filter. After several washes, the RNA was eluted using elution buffer.
Label Biotin
Label protocol standard as recommended by illumina
 
Hybridization protocol standard as recommended by illumina
Scan protocol standard as recommended by illumina
Description Sciatic Nerve, Female and Male, Post natal day 14, Tissue was collected from control littermates
Data processing The data was preprocessed by Bioconductor lumi package (version 1.8.3). It was vst transformed and quantile normalized.
 
Submission date Jun 08, 2009
Last update date Jun 04, 2010
Contact name Beth Yun
E-mail(s) b-yun@northwestern.edu
Organization name Northwestern University
Department Neurology and The Center for Genetic Medicine
Lab Raj Awatramani's Lab
Street address 303 E. Superior St.
City Chicago
State/province IL
ZIP/Postal code 60563
Country USA
 
Platform ID GPL6887
Series (1)
GSE16741 Gene Expression in Mutant P0 Cre Dicer Mouse Sciatic Nerves

Data table header descriptions
ID_REF
nuID nucleotide universal IDentifier (nuID), convertible to and from probe sequence. See Bioconductor lumi package for more details.
VALUE normalized signal intensity
RAW_VALUE raw signal intensity
BEAD_STDERR the standard error of the probe measurements
Detection_Pval the detection p-value of the probe
Avg_NBEADS Number of beads for the probe

Data table
ID_REF nuID VALUE RAW_VALUE BEAD_STDERR Detection_Pval Avg_NBEADS
ILMN_2417611 T.kvUVfFyruaCeqZVs 8.0385644226937 93.51234 3.619987 0.9764957 52
ILMN_2762289 Q96MoiqM_z49FU37tE 8.12345697267794 115.488 4.761553 0.5448718 43
ILMN_2896528 WpaZ9x9f_hAnoR.VBE 12.2670759405659 5651.059 199.6677 0 40
ILMN_2721178 ZhdXp75JftSF3iWLF4 10.4906140040847 1567.286 63.21108 0 39
ILMN_2458837 QoJVn30kwRGJ3SN9Q4 8.09149664663746 107.086 4.286329 0.7649572 46
ILMN_3033922 xnx7ijE2CcOSlY55eA 11.6519867765735 3681.374 112.751 0 43
ILMN_3092673 xPkUXaq37oR_vSi6HY 13.7529941376424 15483.52 439.7692 0 50
ILMN_1230777 BRRdqrfuhH69KLodsc 13.2196213679584 10877.08 308.0482 0 58
ILMN_1246069 upNer6bJTpt27XeZe4 10.8181365336040 2017.187 58.33197 0 67
ILMN_1232042 HiXiBc.AEEDVECwR9M 8.05596266104772 97.8316 4.560646 0.9305556 47
ILMN_1243193 KP_V4grp3bhlw1xJ1U 10.2632932541206 1307.508 49.29468 0 46
ILMN_2524361 ooevXuTfR0trfSs0RE 8.08879588466125 106.3605 3.75647 0.7767094 57
ILMN_1242440 reXYfV2yxHgihX6JJ0 8.1836969524143 131.4539 5.933409 0.2692308 42
ILMN_1233188 Hjr4TIeNxfRUVQuL3k 9.63153544976064 766.1924 24.33104 0 48
ILMN_2543688 xg6JKiieCIm6eohOCE 12.7687613202545 8075.581 189.673 0 42
ILMN_1259789 fdE13SX7F7pLpSCZIA 8.33262842157665 173.3858 6.378376 0.0448718 49
ILMN_2816356 3rtRBd9AcK1Qq07fGI 8.47148771902268 215.3684 13.49294 0.006410256 32
ILMN_1224596 NukioOhS9QN37t2IE4 8.82451034436806 338.2794 11.04951 0 62
ILMN_1233643 3SwZC0nkLddPTf5UEY 8.2089031850566 138.4216 7.073502 0.2040598 45
ILMN_2808939 ZqRKnn0K14kTIep1.c 11.3076750798723 2884.875 129.2415 0 38

Total number of rows: 45281

Table truncated, full table size 3419 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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