|
| Status |
Public on Jun 04, 2010 |
| Title |
control_littermates_WT2 |
| Sample type |
RNA |
| |
|
| Source name |
control_littermates_WT
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Sciatic Nerve
gender: female and male
age: Post natal day 14
genotype: control littermates
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Tissue was collected from control littermates
We used the miRVana miRNA isolation kit from Ambion®. We used the protocol to extract total RNA. Briefly, fresh frozen sciatic nerves were homogenization in lysis buffer followed by a phenol/chloroform extraction. The sample was then combined with 1.25 volumes of 100% ethanol, and passed through a filter. After several washes, the RNA was eluted using elution buffer.
|
| Label |
Biotin
|
| Label protocol |
standard as recommended by illumina
|
| |
|
| Hybridization protocol |
standard as recommended by illumina
|
| Scan protocol |
standard as recommended by illumina
|
| Description |
Sciatic Nerve, Female and Male, Post natal day 14, Tissue was collected from control littermates
|
| Data processing |
The data was preprocessed by Bioconductor lumi package (version 1.8.3). It was vst transformed and quantile normalized.
|
| |
|
| Submission date |
Jun 08, 2009 |
| Last update date |
Jun 04, 2010 |
| Contact name |
Beth Yun |
| E-mail(s) |
b-yun@northwestern.edu
|
| Organization name |
Northwestern University
|
| Department |
Neurology and The Center for Genetic Medicine
|
| Lab |
Raj Awatramani's Lab
|
| Street address |
303 E. Superior St.
|
| City |
Chicago |
| State/province |
IL |
| ZIP/Postal code |
60563 |
| Country |
USA |
| |
|
| Platform ID |
GPL6887 |
| Series (1) |
| GSE16741 |
Gene Expression in Mutant P0 Cre Dicer Mouse Sciatic Nerves |
|
