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Sample GSM414785 Query DataSets for GSM414785
Status Public on Jun 09, 2009
Title lung_AIRmax_male_urethane_mice2
Sample type RNA
 
Source name lung from AIRmax male treated with urethane
Organism Mus musculus
Characteristics tissue: lung
strain: AIRmax
gender: M
agent: urethane
Treatment protocol two intraperitoneal injections of 1 mg/g body weight urethane or saline solution
Extracted molecule total RNA
Extraction protocol Total RNA was extracted with RNeasy Midi kit (Qiagen, Valencia, CA) from normal lung parenchyma and purified with RNeasy MinElute CleanUP Kit (Qiagen). RNA concentration was determined with a Nanodrop spectrophotometer (NanoDrop, Wilmington, DE) and RNA quality was assessed with an Agilent 2100 Bioanalyzer (Agilent Technologies Inc., Santa Clara, CA)
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description no additional information
Data processing The data were normalised using cubic spline normalization with Illumina's BeadStudio Gene Expression Module v3.4, July 2008 (see VALUE column below).
The data were then filtered by detection p-value (p<0.05). A matrix of the normalized, filtered data is linked as supplementary file at the foot of the Series record.
 
Submission date Jun 09, 2009
Last update date Jun 09, 2009
Contact name Loris De Cecco
E-mail(s) loris.dececco@istitutotumori.mi.it
Organization name IRCSS Istituto Nazionale Tumori
Street address via Venezian 1
City Milan
ZIP/Postal code 20133
Country Italy
 
Platform ID GPL6885
Series (1)
GSE16510 Normal lung transcriptome distinguishes mouse lines with different susceptibility to inflammation and to tumorigenesis

Data table header descriptions
ID_REF
VALUE Bead Studio calculated average signal (cubic spline normalized)
Detection Pval BeadStudio Detection p-value

Data table
ID_REF VALUE Detection Pval
ILMN_2896528 15022.83 0
ILMN_2721178 3056.399 0
ILMN_3033922 4702.843 0
ILMN_3092673 18336.54 0
ILMN_2816356 502.5693 0.001253133
ILMN_2808939 3262.042 0
ILMN_2634564 1372.601 0
ILMN_2737647 230.5096 0.1942356
ILMN_2734484 1368.246 0
ILMN_2952292 389.5824 0.01378446
ILMN_2699078 149.964 0.8609022
ILMN_1213681 1650.488 0
ILMN_2735413 208.0443 0.3295739
ILMN_2735415 285.1936 0.0764411
ILMN_2891688 2811.709 0
ILMN_2637698 6362.549 0
ILMN_2674228 1499.581 0
ILMN_2601546 257.4123 0.1203008
ILMN_1230831 351.3154 0.02756892
ILMN_2848071 402.4442 0.0112782

Total number of rows: 25697

Table truncated, full table size 720 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table
Processed data are available on Series record

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