|
| Status |
Public on Jun 10, 2009 |
| Title |
Pancreatic Sample 16-Normal |
| Sample type |
RNA |
| |
|
| Source name |
PancreaticSample16-Normal
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Normal Tissue in Pancreatic Cancer Sample
sex: Male
age: 51
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA extraction was performed as Affymetrix GeneChip expression technical manual (Affymetrix, Inc., Santa Clara, CA). Briefly, total RNA samples were extracted, followed by measurement of the A2260/a280 ratio with at least 1.8 for pure RNA. Quality of the RNA was checked by an Agilent 2100 Bioanalyzer. The Bioanalyzer gel profile exhibited a 28S band that is 2 times more intense than 18S ribosomal RNA. The quality of RNA was assessed by agarose gel electrophoresis.
|
| Label |
biotin
|
| Label protocol |
Lableing was performed according to Affymetrix Gene Chip technical manual
|
| |
|
| Hybridization protocol |
The targets for Affymetrix DNA microarray analysis were prepared according to the manufacturer’s instructions. Biotin-labeled cRNA, produced by in vitro transcription, was fragmented and hybridized to Affymetrix GeneChip Human Genome U133 Plus 2.0 Arrays at 45°C for 16 hr and then washed and stained using the GeneChip Fluidics.
|
| Scan protocol |
The arrays were scanned by a GeneArray Scanner and patterns of hybridization detected as light emitted from the fluorescent reporter groups incorporated into the target and hybridized to oligonucleotide probes.
|
| Description |
Gene expression of -PancreaticSample16-Normal
|
| Data processing |
The data were analyzed with Agilent Gene Spring GX 7.3 version using Affymetrix default analysis settings and GC-RMA as normalization method.
|
| |
|
| Submission date |
Jun 09, 2009 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Liewei Wang |
| E-mail(s) |
wang.liewei@mayo.edu
|
| Phone |
507-284-5264
|
| Fax |
507-284-4455
|
| URL |
http://mayoresearch.mayo.edu/staff/wang_l2.cfm
|
| Organization name |
Mayo Clinic
|
| Department |
Molecular Pharmacology and Experimental Therapeutics
|
| Street address |
200 First street SW
|
| City |
Rochester |
| State/province |
MN |
| ZIP/Postal code |
55905 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE16515 |
Expression data from Mayo Clinic Pancreatic Tumor and Normal samples |
|
| Relations |
| Reanalyzed by |
GSE119087 |
