|
Status |
Public on Jun 10, 2009 |
Title |
TIL #59 CD8+CD28+ |
Sample type |
RNA |
|
|
Source name |
CD8+CD28+
|
Organism |
Homo sapiens |
Characteristics |
cell type: human melanoma tumor infiltrating lymphocytes
|
Treatment protocol |
FACS sorting
|
Growth protocol |
TIL was isolated out of melanoma tumor fragment during 5 weeks culture in presence of IL-2; then subjected to rapid expansion protocol where CD3 stimulation and high dose IL-2 provided; TIL were harvested and sorted based on their CD8+ and CD28 expression. Isolation (5 weeks) -> expansion (2 weeks) -> sorting.
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
|
|
Hybridization protocol |
Standard Illumina hybridization protocol
|
Scan protocol |
Standard Illumina scanning protocol
|
Description |
RNA from sorted TIL sample
|
Data processing |
The data were normalised using BeadStudio software from Illumina
|
|
|
Submission date |
Jun 09, 2009 |
Last update date |
Jun 09, 2009 |
Contact name |
yufeng li |
E-mail(s) |
yufenli@mdanderson.org
|
Phone |
7135630696
|
Organization name |
MDACC
|
Department |
Melanoma
|
Street address |
7455 Fannin
|
City |
houston |
State/province |
TX |
ZIP/Postal code |
77054 |
Country |
USA |
|
|
Platform ID |
GPL6884 |
Series (1) |
GSE16517 |
Human CD28+/- tumor infiltrating lymphocytes gene expression |
|