|
| Status |
Public on Jan 28, 2010 |
| Title |
ES2 clear cell culture, subconfluent |
| Sample type |
RNA |
| |
|
| Source name |
ES2 clear cell culture, subconfluent
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: Ovarian cancer (clear cell) cell line, ES2
|
| Growth protocol |
Primary cultures of normal ovarian surface epithelium (NOSE) were performed as previously described. Briefly, brushings of the ovarian surface were performed in pre- and postmenopausal women undergoing clinically indicated surgery. NOSE cells were cultured in 1:1 MCDB105/M199 (Sigma). The epithelial origin of cultured cells was confirmed using immunohistochemistry to examine the expression of vimentin and cytokeratins, and only cultures containing >90% epithelial cells were used. ES-2 cells were cultured in DMEM/F12 (Invitrogen). TOV-21G cells were cultured in 1:1 MCDB105/M199 (Sigma). RMG-I cells were cultured in Ham’s F-12 (Invitrogen). OVMANA, OVISE, OVAS, OVTOKO, OVSAYO, KK, and SMOV-2d cells were cultured in RPMI 1640 (Invitrogen). NOSE and cancer cell lines were cultured with 10% heat-inactivated fetal bovine serum and 1% penicillin-streptomycin solution (Invitrogen).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the mirVana miRNA Isolation Kit (ABI). RNA quality were inspected on a 2100 Bioanalyzer (Agilent).
|
| Label |
biotin
|
| Label protocol |
According to Illumina protocol.
|
| |
|
| Hybridization protocol |
According to Illumina protocol.
|
| Scan protocol |
According to Illumina protocol.
|
| Description |
Ovarian cancer (clear cell) cell line, ES2
|
| Data processing |
The image data were compiled into a project using Beadstudio software gene expression module version 3.2.7. No normalization was used. The data was exported as txt files using the build-in function of export to GeneSpring GX format. The option of sample probe profile was chosen. Data were subsequently quantile normalized for the analysis.
|
| |
|
| Submission date |
Jun 12, 2009 |
| Last update date |
Jan 28, 2010 |
| Contact name |
Chad Creighton |
| E-mail(s) |
creighto@bcm.tmc.edu
|
| Organization name |
Baylor College of Medicine
|
| Department |
Biostatistics, Ducan Cancer Center
|
| Street address |
One Baylor Plaza, Mail Stop: BCM305
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
|
| Platform ID |
GPL6947 |
| Series (2) |
| GSE16570 |
Gene expression analyses of ovarian clear cell cancer cell lines |
| GSE16574 |
Gene expression analyses of putative miRNA targets in ovarian clear cell cancer cell lines |
|
