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Sample GSM416754 Query DataSets for GSM416754
Status Public on Aug 25, 2009
Title U2OS
Sample type SRA
 
Source name cell line
Organism Homo sapiens
Characteristics cell line: U2OS
Growth protocol RNA was harvested from exponentially growing cells
Extracted molecule total RNA
Extraction protocol Total RNA extracted using standard Trizol extraction. Total RNA fractionated on 15% polyacrylamide gel and 3' and 5' adaptors sequentially ligated requiring a 3' hydroxyl group and 5' phosphate
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection size fractionation
Instrument model Illumina Genome Analyzer
 
Description none
Data processing Reads containing linker matches were mapped (after removing linkers) to predicted pre-miRNAs (MirBase version 12.0)
 
Submission date Jun 12, 2009
Last update date May 15, 2019
Contact name Christine Mayr
E-mail(s) mayrc@mskcc.org
Organization name Whitehead Institute
Street address 9 Cambridge Center
City Cambridge
State/province MA
ZIP/Postal code 02142
Country USA
 
Platform ID GPL9052
Series (1)
GSE16579 miRNA expression of human cancer and mouse cell lines
Relations
SRA SRX012256
BioSample SAMN00004409

Data table header descriptions
SEQUENCE
COUNT counts

Data table
SEQUENCE COUNT
AGCGTGTAGGGATCCAAATCGTATGCCGTCTTCTGC 697906
TAGCTTATCAGACTGATGTTGACTCGTATGCCGTCT 383847
AGCGTGTAGGGATCCAAATCGTATGCCGTCTTCTGT 379730
GGCATTAACGCGGCCGCTCTACAATAGTGATCGTAT 191990
TTAACGCGGCCGCTCTACAATAGTGATCGTATGCCG 180253
TAGCTTATCAGACTGATGTTGATCGTATGCCGTCTT 161703
CATTAACGCGGCCGCTCTACAATAGTGATCGTATGC 53799
AGCGTGTAGGGATCCAAATCGTATGCCGTCTTTTGT 46728
AGCGTGTAGGGATCCAAATCGTATGCCGTCTTCTTT 33454
TAGCTTATCAGACTGATGTTGACATCGTATGCCGTC 33003
TGAGGTAGTAGGTTGTATAGTTTCGTATGCCGTCTT 31314
GCGTGTAGGGATCCAAATCGTATGCCGTCTTCTGCT 27908
TAGCAGCACGTAAATATTGGCGTCGTATGCCGTCTT 25553
TTCAAGTAATCCAGGATAGGCTTCGTATGCCGTCTT 25163
CAGTGCAATGTTAAAAGGGCATTCGTATGCCGTCTT 24902
AGCAGCATTGTACAGGGCTATGATCGTATGCCGTCT 22536
TGGCTCAGTTCAGCAGGAACAGTCGTATGCCGTCTT 21896
AAGCTGCCAGTTGAAGAACTGTTCGTATGCCGTCTT 20584
AGCGTGTAGGGATCCAAATCGTATGCCGTCTTCTTC 20395
TAGCTTATCAGACTGATGTTGACTCGTATGCCTTCT 17742

Total number of rows: 571411

Table truncated, full table size 21784 Kbytes.




Supplementary data files not provided
SRA Run SelectorHelp
Processed data included within Sample table
Raw data are available in SRA
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