|
| Status |
Public on Jun 10, 2010 |
| Title |
HES2-P99+20 +ASC |
| Sample type |
RNA |
| |
|
| Source name |
Embryonic Stem Cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Embryonic Stem Cells
age: 20 passages
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA samples were prepared using Qiagen RNeasy Micro Kit (Qiagen) and then assayed using the Agilent Systems Bioanalyzer 2100 to ensure that high-quality RNA was used for the array experiments.
|
| Label |
Cy3
|
| Label protocol |
Targets were prepared from 200 ng total RNA using Illumina TotalPrep RNA Amplification Kit (Ambion, catalog #AMIL1791)
|
| |
|
| Hybridization protocol |
llumina Human 6 BeadChips (V3) were hybridized, washed, stained and scanned according to Illuminas protocol
|
| Scan protocol |
Processed arrays were read using Illumina BeadReader according to the manufacturer's instructions.
|
| Description |
HES2 HESC P99+20 in KOSR (includes ASC)
|
| Data processing |
Data were first extracted with Illumina BeadStudio software without normalisation. Data was then uploaded into R, where RMA background adjustment (affy package) and Quantile normalisation (normalize.quantiles) was performed
|
| |
|
| Submission date |
Jun 12, 2009 |
| Last update date |
Jun 12, 2009 |
| Contact name |
Sean Grimmond |
| E-mail(s) |
s.grimmond@imb.uq.edu.au
|
| Organization name |
University of Queensland
|
| Department |
Institute for Molecular Bioscience
|
| Lab |
Expression Genomics Lab
|
| Street address |
Services Rd
|
| City |
St Lucia |
| State/province |
Q |
| ZIP/Postal code |
4072 |
| Country |
Australia |
| |
|
| Platform ID |
GPL6884 |
| Series (1) |
| GSE16590 |
Ascorbate effect on serum free cultured hESC |
|
