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Sample GSM4176447 Query DataSets for GSM4176447
Status Public on Jul 06, 2020
Title w1118_Male_4hr_1
Sample type SRA
 
Source name Brain
Organism Drosophila melanogaster
Characteristics strain: w1118
Sex: male
treatment: traumatic brain injury
time point: 4hr
Extracted molecule total RNA
Extraction protocol Direct-zolä RNA MicroPrep kit
QuantSeq 3' mRNA-Seq Library Prep Kit FWD (low-input protocol and 15 ng RNA input)
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NovaSeq 6000
 
Data processing After de-multiplexing with Illumina’s CASAVA 1.8.2 software, the 50 bp reads were aligned to the Drosophila genome (Build dm3) with STAR_2.4 and tabulated for each gene region.
Differential gene expression analysis was used to compare transcriptome changes between conditions using edgeR v.3.22.3 and transcripts were defined as significantly differentially expressed at absolute Log2 fold change >1, FDR <0.05
Genome_build: Dm3
Supplementary_files_format_and_content: Differential gene expression and counts data.xlsx
 
Submission date Nov 19, 2019
Last update date Jul 06, 2020
Contact name Ekta J Shah
E-mail(s) shahekta14@gmail.com
Organization name Wayne State University
Street address 275 E. Hancock
City Detroit
State/province Michigan
ZIP/Postal code 48201
Country USA
 
Platform ID GPL25244
Series (1)
GSE140663 Drosophila Exhibit Divergent Sex-Based Responses in Transcription and Motor Function After Traumatic Brain Injury
Relations
BioSample SAMN13327437
SRA SRX7182887

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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