|
| Status |
Public on Aug 10, 2009 |
| Title |
HS_CD4_Act_18h_ H3K27me1 |
| Sample type |
SRA |
| |
|
| Source name |
CD4+ T cells
|
| Organism |
Homo sapiens |
| Characteristics |
antigen: H3K27me1
antibody: Up 07-448
|
| Treatment protocol |
For activation, anti-CD3/28 beads (Invitrogen) were added to the CD 4 cells for 18h, according to manufacturers protocol. Approximately 86-94% of cells were CD25 positive after the treatment.
|
| Growth protocol |
Human enriched lympocyte fraction was obtained by apheresis. CD4 cells were purified negative purification with kits from Invitrogen or Myltenyi according to manufacturer's protocol.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
After ChIP the libraries were prepared by standard Illumina protocol
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina Genome Analyzer |
| |
|
| Description |
Chromatin IP against H3K27me1
|
| Data processing |
Sequenced 25-bp reads were mapped to the human genome (hg18) using the Solexa Analysis Pipeline, and only those reads that mapped to unique genomic locations with at most 2 mismatches were retained.
|
| |
|
| Submission date |
Jun 17, 2009 |
| Last update date |
May 15, 2019 |
| Contact name |
Keji Zhao |
| E-mail(s) |
zhaok@nhlbi.nih.gov
|
| Fax |
(301) 480-0961
|
| Organization name |
NHLBI, NIH
|
| Lab |
Laboratory of Molecular Immunology
|
| Street address |
Rockville Pike
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL9052 |
| Series (1) |
| GSE16657 |
Chromatin Poises miRNA- and Protein-coding Genes for Expression |
|
| Relations |
| SRA |
SRX012396 |
| BioSample |
SAMN00004723 |
| Named Annotation |
GSM417824_Act-CD4-H3K27me1.bed.gz |
