|
| Status |
Public on Dec 24, 2009 |
| Title |
CyclosporinA_day4_replicate1 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
back skin, day4 after CyclosporinA treatment
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57/BL
tissue: back skin
agent: CyclosporinA
time: 4 days
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Isogen following manufacturer's instructions
|
| Label |
Cy5
|
| Label protocol |
Double-stranded cDNA was synthesized from the 500ng of total RNA, and then cyanin3- or 5- labeled cRNA was made from the cDNA by an in vitro transcription according to the manufacturer’s instruction (Agilent Technologies, Palo Alto, California).
|
| |
|
| Channel 2 |
| Source name |
day4 after ethanol treatment
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57/BL
tissue: back skin
agent: control
time: 4 days
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Isogen following manufacturer's instructions
|
| Label |
Cy3
|
| Label protocol |
Double-stranded cDNA was synthesized from the 500ng of total RNA, and then cyanin3- or 5- labeled cRNA was made from the cDNA by an in vitro transcription according to the manufacturer’s instruction (Agilent Technologies, Palo Alto, California).
|
| |
|
| |
| Hybridization protocol |
Oligoarray control targets and hybridization buffer (Agilent In Situ Hybridization Kit Plus) were added, and samples were applied to microarrays. After hybridization, slides were washed sequential
|
| Scan protocol |
Scanned on an Agilent G2565AA scanner.
|
| Description |
Biological replicate 1 of 2. Day4 after Ciclosporin A treatment. Vs. ethanol treatment
|
| Data processing |
Agilent Feature Extraction Software (v 9.1) was used for background subtraction and LOWESS normalization.
|
| |
|
| Submission date |
Jun 22, 2009 |
| Last update date |
Jun 22, 2009 |
| Contact name |
Yumiko Tsuji |
| E-mail(s) |
yumiko.tsuji@to.shiseido.co.jp
|
| Organization name |
Shiseido
|
| Street address |
2-12-1, Fukuura, Kanazawa-ku
|
| City |
Yokohama |
| ZIP/Postal code |
2368643 |
| Country |
Japan |
| |
|
| Platform ID |
GPL4134 |
| Series (1) |
| GSE16739 |
Murine Back Skin: Control vs. Cyclosporin A-treated |
|
