|
| Status |
Public on Jun 23, 2010 |
| Title |
RAW 264.7, RANKL + M-CSF, miR-155 |
| Sample type |
RNA |
| |
|
| Source name |
RAW 264.7, RANKL + M-CSF, miR-155
|
| Organism |
Mus musculus |
| Characteristics |
cell line: RAW 264.7
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy (Qiagen)
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol for the Mouse 1.0ST arrays from 100ng total RNA.
|
| |
|
| Hybridization protocol |
As per standard Affymetrix protocol for the Mouse 1.0ST arrays.
|
| Scan protocol |
Arrays were scanned on a DNA microarray Agilent Scanner
|
| Description |
osteoclast differentiation of RAW 264.7 transfected with miR-155 expressing vector
|
| Data processing |
Quantile normalisation and Robust Multichip Average (RMA) background correction adjusting for probe sequence (Partek Software) was used to generate gene expression levels from the Mouse Gene 1.0ST arrays.
|
| |
|
| Submission date |
Jun 23, 2009 |
| Last update date |
Jun 25, 2009 |
| Contact name |
Omer Barad |
| E-mail(s) |
omer.barad@weizmann.ac.il
|
| Organization name |
Weizmann Institute of Science
|
| Street address |
Herzel
|
| City |
Rehovot |
| ZIP/Postal code |
76100 |
| Country |
Israel |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE16749 |
miR-155 role in osteoclast differentiation |
|
